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Insulin-like growth factor-binding protein-1 (IGFBP-1) in goldfish, Carassius auratus: molecular cloning, tissue expression, and mRNA expression responses to periprandial changes and cadmium exposure

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Abstract

In this study, the cDNA encoding insulin-like growth factor-binding protein-1 (IGFBP-1) was cloned from the liver of goldfish (Carassius auratus). The obtained goldfish IGFBP-1 cDNA sequence was 1037 bp in length and had an open reading frame of 789 bp encoding a predicted polypeptide of 262 amino acid residues. IGFBP-1 transcript was detected in all tested central nervous and peripheral tissues. The relatively higher levels of IGFBP-1 mRNA were observed in the liver, gill, kidney, heart, spleen, fat and testis, while the lower levels were found in all different regions of brain, muscle and intestine. In the skin, IGFBP-1 mRNA expression level was extremely low. The IGFBP-1 mRNA expression level in liver was significantly elevated after feeding. With cadmium exposure for 24 h, IGFBP-1 mRNA expression levels in spleen and liver were significantly increased at different cadmium concentrations ranging from 0.5 to 10 ppm. The results in this study provided the data regarding molecular characteristics and expression patterns of IGFBP-1 in goldfish and showed that the expression of IGFBP-1 mRNA might be associated with metabolic status and heavy metal stress and regulated by metabolic factors and cadmium in fish.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31101878), the Excellent Youth Foundation of Henan Polytechnic University (No. J2014-02), the Dr. Foundation of Henan Polytechnic University (No. B2010-07) and the Youth Backbone Teacher Foundation of Henan Polytechnic University to Dr. Wenbo Chen.

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Authors and Affiliations

  1. Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, 454000, Henan, People’s Republic of China

    Wenbo Chen, Zhen Zhang & Fangfang Yan

  2. Department of Medicine, Huzhou University, Huzhou, 313000, Zhejiang, People’s Republic of China

    Haiyan Dong

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  1. Wenbo Chen
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  2. Zhen Zhang
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  3. Haiyan Dong
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  4. Fangfang Yan
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Correspondence to Wenbo Chen.

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Chen, W., Zhang, Z., Dong, H. et al. Insulin-like growth factor-binding protein-1 (IGFBP-1) in goldfish, Carassius auratus: molecular cloning, tissue expression, and mRNA expression responses to periprandial changes and cadmium exposure. Fish Physiol Biochem 42, 1043–1052 (2016). https://doi.org/10.1007/s10695-015-0195-x

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  • DOI: https://doi.org/10.1007/s10695-015-0195-x

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