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Differential effects of acute and chronic zinc exposure on lipid metabolism in three extrahepatic tissues of juvenile yellow catfish Pelteobagrus fulvidraco

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Abstract

The aim of this study was to determine the potential mechanisms of exposure to waterborne zinc (Zn) on lipid metabolism in three extrahepatic tissues (ovary, muscle and mesenteric adipose tissue) of female yellow catfish Pelteobagrus fulvidraco. Female yellow catfish were chronically exposed to Zn (0.05, 0.35 or 0.86 mg Zn/l; duration of treatment 8 weeks) or acutely exposed to a high level of Zn (4.71 mg Zn/l for 96 h). Following the respective treatment, lipid deposition and mRNA levels of 11 genes (CPT IA, CPT IB, PPARα, PPARγ, SREBP-1, G6PD, 6PGD, FAS, ACCa, ACCb and LPL) involved in lipid metabolism were determined. Waterborne Zn exposure significantly reduced growth performance and lipid content in muscle but had no significant effect on lipid content in ovary and mesenteric adipose tissue. The change in the levels of the mRNA genes under study was Zn concentration-dependent and tissue-dependent. Pearson correlations between the mRNA levels of three transcriptional factors and enzymes in these tissues revealed that variations in gene expression as a result of the different Zn treatments underlay the patterns of lipid metabolism, which in turn affected fat storage and mobilization. To our knowledge, this is the first study to demonstrate the effect of waterborne Zn exposure on lipid metabolism in extrahepatic tissues at the molecular level. These results therefore contribute to our understanding of Zn-induced toxicity in fish.

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Acknowledgments

This work was supported by the Fundamental Research Funds for the Central Universities (Grant Nos. 2009PY007, 2011PY115, 2013PY073), by the Postgraduates Innovation Research Project of Huazhong Agricultural University (Grant No. 2009sc018) and partly by the National Natural Science Foundation of China (Grant No. 31372547).

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Authors and Affiliations

  1. Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery College, Huazhong Agricultural University, Wuhan, 430070, China

    Jia-Lang Zheng, Zhi Luo, Qing-Ling Zhu, Qi-Liang Chen & Wei Hu

  2. Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, China

    Jia-Lang Zheng, Zhi Luo, Qing-Ling Zhu, Qi-Liang Chen & Wei Hu

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  1. Jia-Lang Zheng
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  2. Zhi Luo
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Correspondence to Zhi Luo.

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Zheng, JL., Luo, Z., Zhu, QL. et al. Differential effects of acute and chronic zinc exposure on lipid metabolism in three extrahepatic tissues of juvenile yellow catfish Pelteobagrus fulvidraco . Fish Physiol Biochem 40, 1349–1359 (2014). https://doi.org/10.1007/s10695-014-9929-4

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