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The proteolytic digestive activity and growth during ontogeny of Parachromis dovii larvae (Pisces: Cichlidae) using two feeding protocols

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Abstract

The proteolytic digestive activity and growth of Parachromis dovii larvae during the ontogeny were evaluated in a recirculation system using two feeding strategies during a 28-day period. Larvae were reared using two feeding protocols (three replicates each): (A) Artemia nauplii (at satiation), fed from exogenous feeding [8 days after hatching (DAH)] until 15 DAH followed by nauplii substitution by formulated feed (20 % day−1) until 20 DAH and then formulated feed until 28 DAH; (B) formulated feed (100 % BW daily) from exogenous feeding until 28 DAH. Levels of acid (pepsin type) and alkaline digestive proteases as well as growth and survival of larvae were measured along the feeding period. Survival was high and similar between treatments: 98.9 ± 0.0 for Artemia, 97.3 ± 0.0 % for formulated feed. The specific growth rate for length and weight was higher in larvae fed with Artemia nauplii than in larvae reared with formulated feed: 3.4 ± 0.1 versus 1.8 ± 0.1 % day−1 for body length (P = 0.009) and 12.2 ± 0.1 versus 6.5 ± 0.3 % day−1 for body weight (P = 0.002). The acid and alkaline proteolytic activity was detected, in both treatments, from the beginning of the experiment, at 8 DAH. The total enzymatic activity (U larva−1) for acid and alkaline proteases was higher in larvae reared with Artemia after 12 DAH, whereas the specific enzymatic activity was similar for both enzyme types in the two treatments. The results suggest that P. dovii larvae were capable to digest formulated diets from the beginning of exogenous feeding and that they could be reared with formulated feeds. However, the formulated feed used should be nutritionally improved because of the poor growth obtained in this research.

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Acknowledgments

This research was supported by the project PS Tecnología Acuicultural of the Escuela de Ciencias Biológicas, Universidad Nacional. We acknowledge the help of Dr. Jorge Alfaro M. for reviewing the manuscript.

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  1. Escuela de Ciencias Biológicas, Universidad Nacional, 86-3000, Heredia, Costa Rica

    José R. Quirós Orlich, Silvia Valverde Chavarría & Juan B. Ulloa Rojas

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  1. José R. Quirós Orlich
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  2. Silvia Valverde Chavarría
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  3. Juan B. Ulloa Rojas
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Correspondence to Juan B. Ulloa Rojas.

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Quirós Orlich, J.R., Valverde Chavarría, S. & Ulloa Rojas, J.B. The proteolytic digestive activity and growth during ontogeny of Parachromis dovii larvae (Pisces: Cichlidae) using two feeding protocols. Fish Physiol Biochem 40, 1253–1261 (2014). https://doi.org/10.1007/s10695-014-9920-0

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