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Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)

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Abstract

Major histocompatibility complex (MHC) plays an important role in the immune response to antigenic peptides in vertebrates. In this study, the full length of MHC IIB cDNA was isolated from the Whitespotted bambooshark (Chiloscyllium plagiosum) by homology cloning, and the rapid amplification of cDNA ends polymerase chain reaction. As a result, the MHC IIB cDNA is 1,407 bp, which contains an open reading frame (ORF) of 831 bp encoding a protein of 276 amino acids. Furthermore, seven alleles of the complete MHC IIB ORF were detected and the variable sites were mainly located in the immunoglobulin-like (β2) region. Tissue distribution analysis showed that MHC IIB can be detected in all the ten tissues examined, with the highest expression in the spleen and gill. Challenge of C. plagiosum with the pathogenic bacteria, Vibrio harveyi, resulted in significant changes in the expression of MHC IIB mRNA in the three immune-related tissues (gill, liver and spleen). These results show that the MHC IIB plays an important role in response to bacterial infection in elasmobranches.

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Acknowledgments

The research was financially supported by the National Natural Science Foundation of China (Grant No.: 40876080).

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Authors and Affiliations

  1. Key Laboratory for Fishery Resources and Eco-environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Qian Ma, Zhi-Meng Zhuang & Qi-Sheng Tang

  2. College of Oceanography and Environmental Science, Xiamen University, Xiamen, 361005, China

    Qian Ma, Yong-Quan Su & Jun Wang

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  1. Qian Ma
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  2. Yong-Quan Su
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  4. Zhi-Meng Zhuang
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Correspondence to Jun Wang.

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Ma, Q., Su, YQ., Wang, J. et al. Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum). Fish Physiol Biochem 39, 131–142 (2013). https://doi.org/10.1007/s10695-012-9685-2

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