Abstract
The goal of this study was to estimate the single and joint effect of the basal region of chromosome 2 and centromeric region of chromosome 8 on morphological and fruit quality traits in tomato (Solanum lycopersicum). The analysis was performed in a population derived from a cross between Rio Grande of S. lycopersicum and LA1589 of S. pimpinellifolium that segregates for both genomic regions. Four major QTLs were found on chromosome 2 and three on chromosome 8, all of them related with morphological traits. QTLs for fruit shape index, proximal fruit end angle and distal fruit end protrusion showed epistatic interaction. Both genomic regions (fs2.1 and fs8.1) explained 62, 47 and 46 % of the phenotypic variability for fruit shape index, proximal fruit end angle and distal fruit end protrusion respectively. Minor QTLs were detected for other morphological and quality traits such as color, pH and fruit shelf life on chromosomes 2 and 8. Only single genomic region effects were found for quality trait. On the other hand, fs2.1 and fs8.1 regions control several fruit morphology attributes following a digenic linear additive model with epistatic interactions.
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Abbreviations
- a/b :
-
Color chroma index
- ANOVA:
-
Analysis of variance
- ar :
-
Fruit area
- BC1 :
-
First cycle of backcross
- CAPS:
-
Cleaved amplified polymorphic sequences
- dan20 :
-
Distal fruit end angle
- dblk20 :
-
Distal fruit end blockiness
- f :
-
Firmness
- F3 :
-
Third filial generation
- fs I :
-
Fruit shape index
- fw :
-
Fruit mass
- H2 :
-
Broad sense heritability
- InDel:
-
Insertion/deletion
- L :
-
Lightness color parameter
- LL:
-
Homozygous for Rio Grande alleles
- LP:
-
Heterozygous
- pan20 :
-
Proximal fruit end angle
- pblk20 :
-
Proximal fruit end blockiness
- pH :
-
Hydrogen potential
- PP:
-
Homozygous for LA1589 alleles
- QTL:
-
Quantitative trait loci
- S1 :
-
First selfed generation
- sl :
-
Fruit shelf life
- ssc :
-
Soluble solid content
- ta :
-
Titratable acidity
- tip :
-
Distal fruit end protrusion
- tri20 :
-
Fruit shape triangle
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10681_2016_1689_MOESM1_ESM.jpg
Supplementary material 1 (JPEG 293 kb) Box plot for the markers with significant association and the highest % R2 in the one-way ANOVA for fruit area (ar), fruit shape index I (fs I), proximal fruit end angle (pan20, in °), dital fruit end angle (dan20, in °), and distal fruit end protrusion (tip). Abbreviations: ma ar, m fsI, m pan20, m dan20, and m tip indicates the average values of the population for each trait. a ar_fs2.1, a ar_fs8.1, a fsI_fs2.1, a fsI_fs8.1, a pan20_fs2.1, a pan20_fs8.1, a dan20_fs2.1, a dan20_fs8.1, a tip_fs2.1, and a tip_fs8.1 indicates de additive value for each trait at the genomic regions fs2.1 and f8.1 respectively. LL: homozygous for Rio Grande alleles, LP: heterozygous, PP: homozygous for LA1589 alleles
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Green, G.Y., Pereira da Costa, J.H., Cambiaso, V. et al. Single and joint effect of the basal region of chromosome 2 and centromeric region of chromosome 8 on morphological and fruit quality traits in tomato. Euphytica 210, 327–339 (2016). https://doi.org/10.1007/s10681-016-1689-z
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DOI: https://doi.org/10.1007/s10681-016-1689-z