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Chromosome elimination of hexaploid common wheat mediated by interaction between Chinese Spring cytoplasm and a genetic factor(s) on chromosome arm 1BL of wild emmer

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Abstract

To create recombinant substitution lines from chromosome arm substitution lines (CASLs) of wild emmer, the common wheat cultivar ‘Bethlehem’ (BLH) was crossed with 4 CASLs of group 1 and the resulting F1s were backcrossed with corresponding BLH ditelosomic (DT) lines to produce monotelosomic plants (BC1F1, 2n = 6x = 41 + t). For each cross combination, about 100 BC1F2 plants derived from 47 to 49 BC1F1 plants were analyzed for their chromosome number and constitution. The frequencies of plants with 42 or 41 + t chromosomes in [DT1BS × (BLH × CASL1BL)] progenies were much lower than in crosses involving CASLs 1AS, 1AL and 1BS; while the frequency of [DT1BS × (BLH × CASL1BL)] progeny plants with 41 chromosomes was high. A total of 49 (BLH × CASL1BL) BC1F1 plants could be clearly classified into three types based on chromosome constitution of their 461 BC1F2 progenies. In type I (21 BC1F1 plants), 95 % of BC1F2 plants possessed 42 chromosomes (counting the telosomic chromosome as an entire chromosome). In type III (24 BC1F1 plants), most BC1F2 plants (75.6 %) had 41 chromosomes and no telosomic chromosome. In type II (4 BC1F1 plants), the expected number of ~48 % of BC1F2 plants had the telosomic chromosome, but about 70 % had fewer than the expected 42 chromosomes. All type III BC1F1 plants were confirmed to have experienced elimination of telosomic chromosome 1BS. Observation of BC1F1 seedling mitosis revealed that the elimination of 1BS occurred most probably during embryogenesis. However, type II BC1F1 plants experienced the elimination of different chromosomes, which was deduced to occur during meiosis. Reciprocal crosses indicated that both types of chromosome elimination occurred only when BLH DT1BS, which has Chinese Spring cytoplasm, was used as female parent. Collectively, these results reveal a genetic system on chromosome arm 1BL of wild emmer that interacts with the CS cytoplasm to cause chromosome elimination.

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References

  • Brasileiro-Vidal AC, Brammer S, Puertas MJ, Zanatta AC, Prestes A, Moraes-Fernandes MIB, Guerra M (2005) Mitotic instability in wheat × Thinopyrum ponticum derivatives revealed by chromosome counting, nuclear DNA content and histone H3 phosphorylation pattern. Plant Cell Rep 24:172–178

    Article  CAS  PubMed  Google Scholar 

  • Endo TR (1978) On the Aegilops chromosomes having gametocidal action on common wheat. In: Proceedings of 5th International conference. Wheat Genetic Symposium. New Delhi 306–314

  • Endo TR (1988) Induction of chromosomal structural changes by a chromosome of Aegilops cylindrica L. in common wheat. J Hered 79:366–370

    Google Scholar 

  • Endo TR (2007) The gametocidal chromosome as a tool for chromosome manipulation in wheat. Chromosome Res 15:67–75

    Article  CAS  PubMed  Google Scholar 

  • Endo TR, Gill BS (1996) The deletion stock of common wheat. J Hered 87:295–307

    Article  CAS  Google Scholar 

  • Feldman M, Liu B, Segal G, Abbo S, Levy AA, Vega JM (1997) Rapid elimination of low-copy DNA sequences in polyploid wheat: a possible mechanism for differentiation of homoeologous chromosomes. Genetics 147:1381–1387

    CAS  PubMed  PubMed Central  Google Scholar 

  • Fu SL, Yang MY, Ren ZL, Yan BJ, Tang ZX (2013a) Abnormal mitosis induced by wheat–rye 1R monosomic addition lines. Genome 57:21–28

    Article  PubMed  Google Scholar 

  • Fu SL, Yang MY, Fei YY, Tan FQ, Ren ZL, Yan BJ, Zhang HY, Tang ZX (2013b) Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines. PLoS ONE 8(7):e70483. doi:10.1371/journal.pone.0070483

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S, Bruss C, Kumlehn J, Matzk F, Houben A (2005) Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell 17:2431–2438

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gupta SB (1969) Duration of mitotic cycle and regulation of DNA replication in Nicotiana plumbaginifolia and a hybrid derivative of N. tabacum showing chromosome instability. Can J Genet Cytol 11:133–142

    Article  Google Scholar 

  • Humphreys MW (1978) Chromosome instability in Hordeum vulgare x H. bulbosum hybrids. Chromosoma 65:301–307

    Article  Google Scholar 

  • Ishii T, Ueda T, Tanaka H, Tsujimoto H (2010) Chromosome elimination by widehybridization between Triticeae or oat plant and pearl millet: pearl milletchromosome dynamics in hybrid embryo cells. Chromosome Res 18:821–831

    Article  CAS  PubMed  Google Scholar 

  • Jin WW et al (2004) Maize centromeres: organization and functional adaptation inthe genetic background of oat. Plant Cell 16:571–581

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Laurie DA, Bennett MD (1989) The timing of chromosome elimination in hexaploid wheat x maize crosses. Genome 32:953–961

    Article  Google Scholar 

  • Liu B, Vega JM, Segal G, Abbo S, Rodova H, Feldman M (1998) Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. I. Changes in low-copy noncoding DNA sequences. Genome 41:272–277

    Article  CAS  Google Scholar 

  • Millet E, Rong JK, Qualset CO, McGuire PE, Bernard M, Sourdille P, Feldman M (2013) Production of chromosome-arm substitution lines of wild emmer in common wheat. Euphytica 190:1–17

    Article  Google Scholar 

  • Munkvold JD, Greene RA, Bermudez-Kandianis CE, La Rota CM, Edwards H, Sorrells SF, Dake T, Benscher D, Kantety R, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorak J, Miftahudin Gustafson JP, Pathan MS, Nguyen HT, Matthews DE, Chao S, Lazo GR, Hummel DD, Anderson OD, Anderson JA, Gonzalez-Hernandez JL, Peng JH, Lapitan N, Qi LL, Echalier B, Gill BS, Hossain KG, Kalavacharla V, Kianian SF, Sandhu D, Erayman M, Gill KS, McGuire PE, Qualset CO, Sorrells ME (2004) Group 3 chromosome bin maps of wheat and their relationship to rice chromosome 1. Genetics 168:639–650

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nasuda S, Friebe B, Gill BS (1998) Gametocidal genes induce chromosome breakage in the interphase prior to the first mitotic cell division of the male gametophyte in wheat. Genetics 149:1115–1124

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pickering RA (1985) Partial control of chromosome elimination by temperature in immature embryos of Hordeum vulgare L. × H. bulbosum. Euphytica 14:869–874

    Article  Google Scholar 

  • Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD, Akhunov ED, Dvorak J, Linkiewicz AM, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis CE, Greene RA, Kantety R, La Rota CM, Munkvold JD, Sorrells SF, Sorrells ME, Dilbirligi M, Sidhu D, Erayman M, Randhawa HS, Sandhu D, Bondareva SN, Gill KS, Mahmoud AA, Ma XF, Miftahudin Gustafson JP, Conley EJ, Nduati V, Gonzalez-Hernandez JL, Anderson JA, Peng JH, Lapitan NL, Hossain KG, Kalavacharla V, Kianian SF, Pathan MS, Zhang DS, Nguyen HT, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Gill BS (2004) A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168:701–712

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rong JK, Millet E, Manisterski J, Feldman M (2000) A new powdery mildew resistance gene: introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115:121–126

    Article  CAS  Google Scholar 

  • Sanei M, Pickering R, Kumke K, Nasuda S, Houben A (2011) Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids. Proc Natl Acad Sci USA 108:11

    Article  Google Scholar 

  • Sears ER and Sears Lotti MS (1978) The telocentric chromosomes of common wheat. In: Proceedings of 5th International Wheat Genetic Symposium, New Delhi 389–407

  • Subrahmanyam NC, Kasha KJ (1973) Selective chromosome elimination during haploid formation in barley following interspecific hybridization. Chromosoma 42:111–112

    Article  Google Scholar 

  • Tsujimoto H, Tsunewaki K (1985) Gametocidal genes in wheat and its relatives. II. Suppressor of the chromosome 3C gametocidal gene of Aegilops triuncialis. Can J Genet Cytol 27:178–185

    Article  Google Scholar 

  • Tu Y, Sun J, Ge X, Li Z (2009) Chromosome elimination, addition and introgression in intertribal partial hybrids between Brassica rapa and Isatis indigotica. Ann Bot 103:1039–1048

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

The research was completed in Professor Moshe Feldman’s laboratory at Weizmann Institute of Science, Israel. Many thanks to him for sharing the data with us and discussion during the manuscript preparing. The manuscript was formed in our current laboratory, which was supported jointly by the National Natural Science Foundation of China (Grant No. 31071421) to Junkang Rong, and the Public Project of Science Technology Department of Zhejiang Province (Grant No. 2014C32027) to Yurong Jiang.

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  1. The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F University, Linan, Hangzhou, 311300, Zhejiang, China

    Y. R. Jiang, M. D. He, M. Q. Ding & J. K. Rong

  2. Department of Plant Sciences, The Weizmann Institute of Science, 761001, Rehovot, Israel

    J. K. Rong

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  1. Y. R. Jiang
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  2. M. D. He
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  3. M. Q. Ding
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Correspondence to J. K. Rong.

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Jiang, Y.R., He, M.D., Ding, M.Q. et al. Chromosome elimination of hexaploid common wheat mediated by interaction between Chinese Spring cytoplasm and a genetic factor(s) on chromosome arm 1BL of wild emmer. Euphytica 209, 615–625 (2016). https://doi.org/10.1007/s10681-016-1634-1

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  • DOI: https://doi.org/10.1007/s10681-016-1634-1

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