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QTL mapping for thermo-sensitive heading date in rice

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Abstract

The main objective of this study was to dissect the genetic basis of rice flowering regulated by temperature. We used two indica varieties Zhenshan 97 and Zhongzao 18, and 168 recombinant inbred lines (RILs) derived from them to estimate temperature effects on heading date (HD). Tests under four different photoperiod and temperature conditions in growth chambers showed that HD of both parents was accelerated at high temperatures, indicating that both parents were strongly thermo-sensitive. The averaged effective cumulative temperatures (ECTs), 1,120 and 1,263 °C necessary for Zhenshan 97 and Zhongzao 18 respectively, could be the prerequisite in a given condition before heading. QTL analysis was conducted with the RILs grown in both long day and short day conditions in 2 years. A total of 4 ECT QTLs corresponding to 4 HD QTLs were mapped. The major ECT QTL, qEHD10, was repeatedly identified on chromosome 10, which could explain 42.2–57.0 % of ECT variation and 39.8–59.4 % of HD variation in four environments. Overall these novel findings would improve the knowledge of temperature on rice flowering.

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Acknowledgments

This work was supported by Grants from the National Natural Science Foundation of China (91335201), National Key Program on Basic Research Projects (2010CB125901) and the Bill & Melinda Gates Foundation.

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    1. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

      Mallikarjuna Rao Kovi, Yong Hu, Xufeng Bai & Yongzhong Xing

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    1. Mallikarjuna Rao Kovi
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    2. Yong Hu
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    Correspondence to Yongzhong Xing.

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    Mallikarjuna Rao Kovi and Yong Hu have contributed equally to this work.

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    Kovi, M.R., Hu, Y., Bai, X. et al. QTL mapping for thermo-sensitive heading date in rice. Euphytica 205, 51–62 (2015). https://doi.org/10.1007/s10681-015-1383-6

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