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An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans

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Abstract

Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.

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Acknowledgments

The authors thank Dirk Jan Huigen and Henk Smid for their technical support. Wageningen UR Plant Breeding is acknowledged for financial support to Suxian Zhu. Geert Kessel, Jack Vossen, Marjan Bergervoet, Linda Kodde and Maarten Nijenhuis were supported by the DuRPh program, granted by the Ministry of Economic Affairs, formerly the Ministry of Agriculture and Fisheries, of the Netherlands.

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Authors and Affiliations

  1. Wageningen UR Plant Breeding, Wageningen University and Research Center, PO Box 386, 6700 AJ, Wageningen, The Netherlands

    Suxian Zhu, Jack H. Vossen, Marjan Bergervoet, Maarten Nijenhuis, Linda Kodde, Vivianne Vleeshouwers, Richard G. F. Visser & Evert Jacobsen

  2. Plant Research International, Biointeractions and Plant Health, Wageningen University and Research Center, PO Box 16, 6700 AB, Wageningen, The Netherlands

    Geert J. T. Kessel

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  1. Suxian Zhu
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  2. Jack H. Vossen
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Correspondence to Jack H. Vossen.

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Zhu, S., Vossen, J.H., Bergervoet, M. et al. An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans . Euphytica 202, 219–234 (2015). https://doi.org/10.1007/s10681-014-1276-0

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