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Parental genome contribution in maize DH lines derived from six backcross populations using genotyping by sequencing

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Abstract

Molecular characterization of doubled haploid (DH) maize lines and estimation of parental genome contribution (PGC) may be useful for choosing pairs of DH lines for hybrid make up and new pedigree starts. Six BC1-derived DH populations created by crossing two donor with three recurrent parents were genotyped with 97,190 polymorphic markers with the objectives of: (i) understanding genetic purity, genetic distance and relationship among 417 maize DH lines; (ii) estimating PGC of the DH lines derived from different genetic backgrounds; and (iii) understanding the correlation between donor parent introgression and testcross performance for grain yield and anthesis-silking interval (ASI) under managed drought and optimum environments. The DH lines were 97 % genetically pure, with <2 % heterogeneity; only two DH lines showed heterogeneity >5 %, which is likely to be due to errors during seed multiplication or maintenance. Genetic distance between pairwise comparisons of the 417 DH lines ranged from 0.055 to 0.457; only 0.01 % showed a genetic distance <0.100, indicating large genetic differences among the DH lines. Both populations 1 and 6 showed significantly lower (p < 0.001) donor introgression than the other four populations. Donor parent contribution was significantly (p < 0.001) higher in the CML444 genetic background than CML395 and CML488. The average donor and recurrent PGC across all 417 DH lines was 31.7 and 64.3 %, respectively. Donor genome introgression was higher than expected in 82 % of the DH lines in the BC1 generation, possibly due to artificial selection during the DH process, during the development of F1 or BC1 seed, or during initial agronomic evaluation of the DH lines. Donor parent introgression up to 32 % showed significant positive correlation with grain yield under drought (r = 0.312, p < 0.001) and optimum (r = 0.142, p < 0.050) environments but negative correlation with ASI under drought (r = −0.276, p < 0.001). Additional multi-environment phenotype data under managed drought are needed to confirm the correlations reported in this study and to map the specific genomic regions associated with such correlations.

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Acknowledgments

DH lines used in this study and their phenotypic data were generated as part of the WEMA project, funded by the Bill & Melinda Gates Foundation. GBS data were generated as part of the Basic Research to Enabling Agricultural Development (BREAD) project, funded by the US National Science Foundation. We are grateful to CIMMYT Field Technicians at the different stations in Kenya for the phenotypic evaluation, and Monsanto Company for developing the DH populations.

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Authors and Affiliations

  1. International Maize and Wheat Improvement Center (CIMMYT), P. O. Box 1041, Village Market, Nairobi, 00621, Kenya

    Veronica Ogugo, Kassa Semagn & Yoseph Beyene

  2. Department of Biochemistry and Biotechnology, Kenyatta University, P. O. Box 43844, Nairobi, 0100, Kenya

    Steven Runo

  3. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, D.F., Mexico

    Michael Olsen

  4. United States Department of Agriculture-Agricultural Research Service, Corn Host Plant Resistance Research Unit, Box 9555, Mississippi, MS, 39762, USA

    Marilyn L. Warburton

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  1. Veronica Ogugo
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  2. Kassa Semagn
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  3. Yoseph Beyene
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  4. Steven Runo
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  5. Michael Olsen
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  6. Marilyn L. Warburton
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Correspondence to Kassa Semagn.

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Ogugo, V., Semagn, K., Beyene, Y. et al. Parental genome contribution in maize DH lines derived from six backcross populations using genotyping by sequencing. Euphytica 202, 129–139 (2015). https://doi.org/10.1007/s10681-014-1238-6

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  • DOI: https://doi.org/10.1007/s10681-014-1238-6

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