Abstract
The physiological basis of genetic variation in drought response and its association with yield and related indices is not clear in tall fescue. In this study thirty genotypes of tall fescue (Festuca arundinacea Schreb.) were sampled from a polycross population and evaluated under two levels of irrigation in 2010 (normal and intense stress) and 2011 (normal and mild stress). Physiological traits including relative water content (RWC), total chlorophyll (TChl), chlorophyll a (Chla), chlorophyll b (Chlb), Chla/Chlb, carotenoids (Car), TChl/Car and proline content along with forage yield, agro-morpholgical traits and selection indices (stress tolerance index, STI and drought susceptibility index, DSI) were studied. Large variation and moderate to high heritability was estimated for most of the studied traits. Intense drought condition decreased chlorophyll content while mild stress significantly increased it. In the other hand intense drought stress increased Chla/b while mild stress didn’t change it. Under mild drought stress condition STI was positively correlated with RWC while under intense drought stress condition STI was positively correlated with chlorophyll content. Although proline content was significantly increased in both intense and mild drought stress conditions, no relationship was found between proline accumulation with forage yield and STI. Applications of principle component analysis for screening suitable genotypes are also discussed.
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References
Abraham EM, Huang B, Bonos SA, Meyers WA (2004) Evaluation of drought resistance for texas bluegrass, kentucky bluegrass, and their hybrids. Crop Sci 44:1746–1753
Allen RG, Pereira LS, Raes D, Smith M (1998) Crop Evapotranspiration: Guidelines for Computing Crop Requirements. FAO Irrigation and Drainage Paper No.56. FAO, Rome, Italy
Andrew JS, Moreau H, Kuntz M, Pagny G, Lin C, Tanksley S, McCarthy J (2008) An investigation of carotenoid biosynthesis in Coffea canephora and Coffea arabica. Plant Physiol 165:1087–1106
Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216
Baquedano FJ, Castillo FJ (2006) Comparative ecophysiological effects of drought on seedlings of the Mediterranean water-saver Pinus halepensis and waterspenders Quercus coccifera and Quercus ilex. Tree struct Funct 20:689–700
Bates LS, Waldren RP, Teare LD (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
Bayat F, Mirlohi A, Khodambashi M (2009) Effects of endophytic fungi on some drought tolerance mechanisms of tall fescue in a hydroponics culture. Russian J Plant Physiol 56:563–570
Blum A (2011) Plant breeding for water limited environments. Springer, New York
Clarke Topp C, Parkin GW, Ferre TPA (2008) Soil water content. In: Carter MR, Gregorich EG (eds) Soil sampling and methods of analysis. Canadian Soc Soil Sci, Pinawa
De-Lacerda CF, Cambraia J, Oliva MA, Ruiz HA, Prisco JT (2003) Solute accumulation and distribution during shoot and leaf development in two sorghum genotypes under salt stress. Environ Exp Bot 49:107–120
Dencic S, Kastori R, Kobiljski B, Duggan B (2000) Evaluation of grain yield and its components in wheat cultivates and landraces under near optimal and drought conditions. Euphytica 113:43–52
Eagles CF, Thomas H, Volaire F, Howarth CJ (1997) Stress physiology and crop improvement. Plant physiol Growth 3:141–150
Ebrahimiyan M, Majidi MM, Mirlohi A, Gheysari M (2012) Drought tolerance indices in a tall fescue population and its polycross progenies. Crop Pasture Sci 63:360–369
Elsheery NI, Cao KF (2008) Gas exchange, chlorophyll fluorescence, and osmotic adjustment in two mango cultivars under drought stress. Acta Physiol Plant 30:769–777
El-Tayeb MA (2006) Differential response of two vicia faba cultivars to drought: growth, pigments, lipid, peroxidation, organic solutes, catalase, and peroxidase activity. Acta Agron Hung 54:25–37
Fernandez GCJ (1992) Effective selection criteria for assessing plant stress tolerance. In: Kuo CC (ed.), Proc. of an international symposium on adaptation of food crops to temperature and water stress. AVRDC, Shanhua, Taiwan. 257–270
Fu J, Huang B (2001) Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45:105–114
Garcı´a-Valenzuela X, Garcı´a-Moya E, Rasco´n-Cruz Q, Herrera-Estrella L, Aguado-Santacruz GA (2005) Chlorophyll accumulation is enhanced by osmotic stress in graminaceous chlorophyllic cells. Plant Physiol 162:650–661
Hallauer AR, Carena MJ, Miranda JB (2010) Quantitative genetics in maize breeding. Iowa state university press, Oxford
Haoz F, Li XH, Su ZJ, Xie CX, Li MS, Liang XL, Weng JF, Zhang DG, Li L, Zhang S (2011) A proposed selection criterion for drought resistance across multiple environments in maize. Breed Sci 61:101–108
Hare PD, Cress WA (1997) Metabolic implications of stress induced proline accumulation in plants. Plant Growth Regul 21:79–102
Hsu SY, Hsu YT, Kao CH (2003) The effect of polyethylene glycol on proline accumulation in rice leaves. Biol Plant 46:73–78
Huang B, Gao H (2000) Root Physiological characteristics associated with drought resistance in tall fescue cultivars. Crop Sci 40:196–203
Hura T, Grzesiak S, Hura K, Thiemtm E, Tokarz K, Wedzony M (2007) Physiological and biochemical tools useful in drought tolerance detection in genotypes of winter triticale: accumulation of Ferulic acid correlates with drought tolerance. Ann Bot 100:767–775
SPSS Inc (2001) Wacker Drive S, Chicago, Illinois, USA
Jaleel CA, Manivannan P, Wahid A, Faros M, Al-juburi HJ, Somasundaram R, Panneerselvam R (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. Int J Agric Biol 11:100–105
Jiang Y, Huang B (2001) Drought and heat stress injury to two cool-season turfgrass in relation to antioxidant metabolism and lipid peroxidation. Crop Sci 41:436–442
Johanson RA, Wichern DW (2007) Applied multivariate statistical analysis. Prentice Hall Inter. Inc, New Jersey
Kavi Kishore PB, Sangam S, Amrutha RN, Laxmi PS, Naidu KR, Rao KRSS, Rao S, Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438
Kearsey MJ, Pooni HS (1996) The Genetical analysis of quantitative traits. Chapman and Hall, New York
Keles Y, Oncel I (2004) Growth and solute composition in two wheat species experiencing combined influence of stress conditions. Russian J Plant Physiol 51:228–233
Kirigwi FM, Van Ginkel M, Trethowan R, Sears RG, Rajaram S, Paulsen GM (2004) Evaluation of selection strategies for wheat adaptation across water regimes. Euphytica 135:361–371
Lichtenthaler HK, Buschmann C (2001) Chlorophylls and Carotenoids: measurement and characterization by UV-VIS spectroscopy. John Wiley and Sons, Inc. New York
Liu C, Liu Y, Guo K, Fan D, Li G, Zheng Y, Yu L, Yang R (2011) Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China. Environ Exp Bot 71:174–183
Maggio A, Miyazaki S, Veronese P, Fujita T, Ibeas JI, Damsz B, Narasimhan ML, Hasegawa PM, Joly RJ, Bressan RA (2002) Does proline accumulation play an active role in stress-induced growth reduction? Plant J 31:699–712
Majidi MM, Mirlohi A, Amini F (2009) Genetic variation, heritability and correlations of agro-morphological traits in tall fescue (Festuca arundinacea Schreb.). Euphytica 167:323–331
Mensah JK, Obadoni BO, Eruotor PG, Onome-Irieguna F (2006) Simulated flooding and drought effects on germination, growth, and yield parameters of sesame (Sesamum indicum L.). Afr J Biotechnol 5:1249–1253
Merewitz E, Meyer W, Bonos S, Huang BR (2010) Drought stress responses and recovery of texas x kentucky hybrids and kentucky bluegrass genotypes in temperate climate conditions. Agron J 102:258–268
Mirlohi A, Sabzalian MR, Khayyam Nekouei M (2004) Endophytic fungi, characteristics and their potential for genetic manipulation. Iran J Biotechnol 2:75–83
Montgomery DC (2006) Introduction to linear regression analysis. Jon Wiley and Sons, New York
Munne-Bosch S, Pe˜nuelas J (2003) Photo- and antioxidative protection during summer leaf senescence in Pistacia lentiscus L. grown under Mediterranean field conditions. Ann Bot 92:385–391
Norton MR, Volaire F, Lelièvre F (2006) Summer dormancy in Festuca arundinacea Schreb.; the influence of season of sowing and a simulated mid-summer storm on two contrasting cultivars. Aust J Agric Res 57:1267–1277
Ramirez Vallejo P, Kelly JD (1998) Traits related to drought resistance in common bean. Euphytica 99:127–136
SAS institute (2001) User’s guide. Release 8.2 SAS Institute, Cary N.C. Nos SAS and SSSA, Madison, W. pp 225–293
Seraj R, Sinclair TR (2002) Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant Cell Environ 25:333–341
Wang JP, Bughrara SS (2008) Evaluation of drought tolerance for Atlas fescue, perennial ryegrass, and their progeny. Euphytica 164:113–122
Wang Z, Huang B (2004) Physiological recovery of kentucky bluegrass from simultaneous drought and heat stress. Crop Sci 44:1729–1736
Weatherley PE (1950) Studies in the water relations of cotton plants I. The field measurement of water deficit in leaves. New Phytol 49:81–87
XiangYong Z, QingHua Y, ShengJiang H (2009) Physiology study of drought resistance of tall fescue in the seedling stage. Southwest China J Agric Sci 22:621–624
Xiao X, Xu X, Yang F (2008) Adaptive responses to progressive drought stress in two populus cathayana populations. Silva Fennica 42(5):705–719
XiaoRong X, Wei L, JinRong L, JianXiong D (2009) Physiological evaluation of drought and heat resistance for 21 tall fescue turfgrass. Acta Agrestia Sinica 17:202–205
Yan H, Gang LZ, Zhao CY, Guo WY (2000) Effects of exogenous proline on the physiology of soyabean plantlets regenerated from embryos in vitro and on the ultrastructure of their mitochondria under NaCl stress. Soybean Sci 19:314–319
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Ebrahimiyan, M., Majidi, M.M., Mirlohi, A. et al. Physiological traits related to drought tolerance in tall fescue. Euphytica 190, 401–414 (2013). https://doi.org/10.1007/s10681-012-0808-8
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DOI: https://doi.org/10.1007/s10681-012-0808-8