Skip to main content

Advertisement

SpringerLink
Log in

Clonal variation and stability assay of chimeric Pinot Meunier (Vitis vinifera L.) and descending sports

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

This project studied the genetic variation of the periclinal grape chimera Pinot Meunier, its natural occurring mutations (loss of trichomes on leaf surfaces) and the German Pinot noir clone Samtrot. Eleven Pinot Meunier clones of French, Italian and foremost German origin, Pinot Meunier mutations of differing ages and with various dispersions of hairless sectors as bud-, shoot- and complete vine mutations and six Samtrot clones were investigated with amplified fragment length polymorphisms (AFLPs) and microsatellites (SSRs). SSR-analysis proved chimerism of all Pinot Meunier clones tested and confirmed identical genotype for all hairless mutations and Samtrot clones at the analyzed loci. Clonal variation was shown by AFLP-analysis yielding a total of 670 bands out of 18 primer combinations of which 161 were polymorphic. Pinot Meunier, Samtrot and the naturally occurring hairless Pinot Meunier mutations could be significantly differentiated. Most of interclonal varying AFLP fragments (mean 1.5% per sample) originated within the groups of Samtrot or Pinot Meunier mutations, whereas intraclonal identity was highest within the Pinot Meunier clones. According to the analysis of molecular variance (AMOVA), variation among wild type and mutated Pinot Meunier leaf halves is significantly smaller than between phenotypically identical Samtrot and Pinot Meunier mutants. Average gene diversity calculated on variability of loci reduced from Samtrot (0.040 ± 0.023) towards Pinot Meunier (0.025 ± 0.013) clones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

AFLP:

Amplified fragment length polymorphism

PCO:

Principal coordinate analysis

SSR:

Simple sequence repeats

AMOVA:

Analysis of molecular variance

References

  • Bellin D, Velasco R, Grando MS (2001) Intravarietal DNA polymorphisms in grapevine (Vitis vinifera L.). Acta Hortic 546:343–349

    CAS  Google Scholar 

  • Bleyer K (2001) Klonenzüchtung beim Blauen Spätburgunder. Rebe Wein 11:22–26

    Google Scholar 

  • Bonin A, Belleman E, Eidesen PB, Pompanon F, Brochmann C, Taberlet P (2004) How to track and asses genotyping errors in population genetics studies. Mol Ecol 13:3261–3273. doi:10.1111/j.1365-294X.2004.02346.x

    Article  PubMed  CAS  Google Scholar 

  • Boss PK, Thomas MR (2002) Association of dwarfism and floral induction with a grape “green revolution” mutation. Nature 416:847–850. doi:10.1038/416847a

    Article  PubMed  CAS  Google Scholar 

  • Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation and characterization of newpolymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39:628–633. doi:10.1139/g96-080

    Article  PubMed  CAS  Google Scholar 

  • Bowers JE, Boursiquot JM, This P, Chu K, Johansson H, Meredith C (1999) Historical genetics: the parentage of Chardonnay, Gamay and other wine grapes of northeastern France. Science 285:1562–1565. doi:10.1126/science.285.5433.1562

    Article  PubMed  CAS  Google Scholar 

  • Breider H (1953) Entwicklungsgeschichtlich-genetische Studien über somatische Mutationen bei der Rebe. Theor Appl Genet 23(7–8):208–222. doi:10.1007/BF00712128

    Google Scholar 

  • Bundessortenamt (2000) Beschreibende Sortenliste—Reben. Deutscher Landwirtschaftsverlag GmbH, Hannover

    Google Scholar 

  • Cameron JW, Soos RK, Olson EO (1964) Chimeral basis for color in pink and red grapefruit. J Hered 55:23–28

    Google Scholar 

  • Chatelet P, Lacou V, Fernandez L, Sreekantan L, Lacombe T, Martinez-Zapater JM et al (2007) Characterization of Vitis vinifera L. somatic variants exhibiting abnormal flower development patterns. J Exp Bot 58:4107–4118. doi:10.1093/jxb/erm269

    Article  PubMed  CAS  Google Scholar 

  • Crespan M (2004) Evidence on the evolution of polymorphism of microsatellite markers in varieties of Vitis vinifera L. Theor Appl Genet 108(2):231–237. doi:10.1007/s00122-003-1419-5

    Article  PubMed  CAS  Google Scholar 

  • Dermen H (1960) Nature of plant sports. Am Hortic Mag 39:123–173

    Google Scholar 

  • Forneck A (2005) Clonality—a concept for stability and variability during vegetative propagation. In: Esser K, Lüttge U, Beyschlag W, Murata J (eds) Progress in Botany, vol 66. Springer-Verlag, Berlin

    Chapter  Google Scholar 

  • Franks T, Botta R, Thomas MR (2002) Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement. Theor Appl Genet 104(2–3):192–199. doi:10.1007/s001220100683

    Article  PubMed  CAS  Google Scholar 

  • Gower JC (1966) Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika 55:582–585. doi:10.1093/biomet/55.3.582

    Article  Google Scholar 

  • Gunther E (1961) Durch Chimärenbildung verursachte Aufhebung der Selbstinkompatibilität von Lycopersicum esculentum L. Mill. Ber Dtsch Bot Ges 74:333–336

    Google Scholar 

  • Hocquigny S, Pelsy F, Dumas V, Kindt S, Heloir M-C, Merdinoglu D (2004) Diversification within grapevine cultivars goes through chimeric states. Genome 47:579–589. doi:10.1139/g04-006

    Article  PubMed  CAS  Google Scholar 

  • Imazio S, Labra M, Grassi F, Winfield M, Bardini M, Scienza A (2002) Molecular tools for clone identification: the case of the grapevine cultivar ‘Traminer’. Plant Breed 121:531–535. doi:10.1046/j.1439-0523.2002.00762.x

    Article  CAS  Google Scholar 

  • Jaillon O, Aury J-M, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C et al (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449:463–467. doi:10.1038/nature06148

    Article  PubMed  CAS  Google Scholar 

  • Klekowski EJ (1998) Mutation rated in mangroves and other plants. Genetica 102–103:325–331. doi:10.1023/A:1017026907407

    Article  Google Scholar 

  • Labra M, Imazio S, Grassi F, Rossoni M, Sala F (2004) Vine-1 retrotransposon-based sequence-specific amplified polymorphism for Vitis vinifera L. genotyping. Plant Breed 123:180–185. doi:10.1046/j.1439-0523.2003.00965.x

    Article  CAS  Google Scholar 

  • Lefort F, Kyvelos CJ, Zervou M, Edwards KJ, Roubelakis-Angelakis KA (2002) Characterization of new microsatellite loci from Vitis vinifera and their conservation on some Vitis species and hybrids. Mol Ecol Notes 2(1):20–21. doi:10.1046/j.1471-8286.2002.00130.x

    Article  CAS  Google Scholar 

  • Marcotrigiano M (1997) Chimeras and variegation: patterns of deceit. HortScience 32(5):773–784

    Google Scholar 

  • Marcotrigiano M (2000) Herbivory could unlock mutations sequestered in stratified shoot apices of genetic mosaics. Am J Bot 87(3):355–361. doi:10.2307/2656631

    Article  PubMed  Google Scholar 

  • Marcotrigiano M, Bernatzky R (1995) Arrangement of cell layers in the shoot apical meristems of periclinal chimeras influences cell fate. Plant J 7(2):193–202. doi:10.1046/j.1365-313X.1995.7020193.x

    Article  Google Scholar 

  • Milbourne D, Meyer R, Bradshaw JE, Baird E, Bonar N, Provan J et al (1997) Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Mol Breed 3(2):1572–1588. doi:10.1023/A:1009633005390

    Article  Google Scholar 

  • Moncada X, Pelsy F, Merdinogul D, Hinrichsen P (2006) Genetic diversity and geographical dispersal in grapevine clones revealed by microsatellite markers. Genome 49:1459–1472. doi:10.1139/G06-102

    Article  PubMed  CAS  Google Scholar 

  • Müller-Stoll WR (1950) Mutative Färbungsänderungen bei Weintrauben. Theor Appl Genet 20:288–291. doi:10.1007/BF00709449

    Article  Google Scholar 

  • Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

    Google Scholar 

  • Pineda-Krch M, Lehtilä K (2004) Cost and benefits of genetic heterogeneity within organisms. J Evol Biol 17:1167–1177. doi:10.1111/j.1420-9101.2004.00808.x

    Article  PubMed  CAS  Google Scholar 

  • Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S et al (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2(3):225–238. doi:10.1007/BF00564200

    Article  CAS  Google Scholar 

  • Pratt C (1959) Radiation damage in shoot apices of concord grape. Am J Bot 46:103–109. doi:10.2307/2439465

    Article  Google Scholar 

  • Regner F, Stadlbauer A, Eisenheld C, Kaserer H (2000) Genetic relationship and related cultivar. Am J Enol Vitic 51:7–14

    CAS  Google Scholar 

  • Riaz S, Garrison KE, Dangl GS, Boursiquot J-M, Meredith CP (2002) Genetic divergence and chimerism within ancient asexually propagated winegrape cultivars. J Am Soc Hortic Sci 127(4):508–514

    CAS  Google Scholar 

  • Rodríguez López CM, Wetten AC, Wilkinson MJ (2004) Detection and quantification of in vitro-culture induced chimerism using simple sequence repeat (SSR) analysis in Theobroma cacao (L.). Theor Appl Genet 110:157–166. doi:10.1007/s00122-004-1823-5

    Article  PubMed  CAS  Google Scholar 

  • Rohlf FJ (2000) NTSYSpc Nummerical taxonomy and multivariate analysis, Version 2.1. Exeter Software, New York

    Google Scholar 

  • Santelices B (2004) Mosaicism and chimerism as components of intraorganismal genetic heterogeneity. J Evol Biol 17:1187–1188. doi:10.1111/j.1420-9101.2004.00813.x

    Article  PubMed  CAS  Google Scholar 

  • Schmidt A (1924) Histologische Studien an phanerogamen Vegetationspunkten. Bot Arch 8:345–404

    Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN, Version 2.000: a software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva, Geneva

    Google Scholar 

  • Scott KD, Ablett EM, Lee LS, Henry RJ (2000) AFLP markers distinguishing an early mutant of flame seedless grape. Euphytica 113:245–249. doi:10.1023/A:1003977408214

    Article  CAS  Google Scholar 

  • Sefc KM, Regner F, Turetschek E, Glössl J, Steinkeller H (1999) Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome 42:367–373. doi:10.1139/gen-42-3-367

    Article  PubMed  CAS  Google Scholar 

  • Skene KGM, Barlass M (1983) Studies on the fragmented shoot apex of grapevine. J Exp Bot 34(147):1271–1280. doi:10.1093/jxb/34.10.1271

    Article  Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical taxonomy. W.H. Freeman and Company, San Francisco

    Google Scholar 

  • Stenkamp S, Ottaviano F, Becker M, Forneck A, Blaich R (2005) Chimären—in verbreitetes Phänomen im Weinbau. In: Schruft G (ed) Deutsches Weinbau-Jahrbuch 2005. Verlag Eugen Ulmer, Stuttgart

    Google Scholar 

  • Stenkamp S, Becker M, Hill B, Blaich R, Forneck A (2007) Genetic variation among chimeric Pinot Meunier clones (Vitis vinifera L.). In: IX international conference on grape genetics and breeding, Udine, Italy, 2–6 July 2006, Acta Hort (in press)

  • Stewart RN, Dermen H (1970) Determination of number and mitotic activity of shoot apical initial cells by analysis of mericlinal chimeras. Am J Bot 57(7):816–826. doi:10.2307/2441339

    Article  Google Scholar 

  • Tajima F (1983) Evolutionary relationship of DNA sequences in finite populations. Genetics 105:437–460

    PubMed  CAS  Google Scholar 

  • Thomas MR, Scott NS (1993) Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed a sequence-tagged sites (STSs). Theor Appl Genet 86:985–990

    CAS  Google Scholar 

  • Thompson MM, Olmo HP (1963) Cytohistological studies of cytochimeric and tetraploid grapes. Am J Bot 50:901–906. doi:10.2307/2439777

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414. doi:10.1093/nar/23.21.4407

    Article  PubMed  CAS  Google Scholar 

  • Walker AR, Lee E, Robinson SP (2006) Two new grape cultivars, bud sports of Cabernet Sauvignon bearing pale-coloured berries are the result of deletion of two regulatory genes of the berry colour locus. Plant Mol Biol 62:623–635. doi:10.1007/s11103-006-9043-9

    Article  PubMed  CAS  Google Scholar 

  • Yakushiji H, Kobayashi S, Goto-Yamamoto N, Tae Jeong S, Sueta T, Mitani N et al (2006) A skin color mutation of grapevine, from black-skinned Pinot noir to white-skinned Pinot blanc, is caused by deletion of the functional VvmybA1 allele. Biosci Biotechnol Biochem 70(6):1506–1508. doi:10.1271/bbb.50647

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank Karl Bleyer (State Institute of Viticulture, Oenology and Fruit Technology, Weinsberg/Germany), Frédérique Pelsy and Didier Merdinoglu (Institute National de la Recherche Agronomique, Université Louis Pasteur, Colmar/France) for scientific discussion, Helga Miehle (University of Natural Resources and Applied Plant Sciences, Vienna/Austria) for careful review of different versions of this paper and Sonja Havrda (University of Hohenheim, Stuttgart/Germany) and Roman Leszkovits (University of Natural Resources and Applied Plant Sciences, Vienna/Austria) for technical assistance. This study contains parts of the master research of M.B. supported by the foreign exchange ‘Duke Carl scholarship’ of the University of Hohenheim and is part of the PhD research of S.S.

Author information

Author notes

  1. Sabine H. G. Stenkamp

    Present address: Institute of Viticulture/Grapevine Breeding/Wine Technology, Section for Grapevine Breeding & Grafting, Geisenheim Research Centre, 65366, Geisenheim, Germany

Authors and Affiliations

  1. Department of Applied Plant Sciences and Plant Biology, Institute of Horticulture and Viticulture, University of Natural Resources and Applied Plant Sciences (BOKU), 1190, Vienna, Austria

    Sabine H. G. Stenkamp, Manuel S. Becker & Astrid Forneck

  2. State Institute for Viticulture, Oenology and Fruit Technology (LVWO), 74189, Weinsberg, Germany

    Bernd H. E. Hill

  3. Institute for Special Crop Cultivation and Crop Physiology, University of Hohenheim, 70593, Stuttgart, Germany

    Rolf Blaich

Authors
  1. Sabine H. G. Stenkamp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manuel S. Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bernd H. E. Hill
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rolf Blaich
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Astrid Forneck
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Astrid Forneck.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stenkamp, S.H.G., Becker, M.S., Hill, B.H.E. et al. Clonal variation and stability assay of chimeric Pinot Meunier (Vitis vinifera L.) and descending sports. Euphytica 165, 197–209 (2009). https://doi.org/10.1007/s10681-008-9807-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-008-9807-1

Keywords

Search

Navigation