Abstract
The meiotic behavior of two half-sib interspecific tetraploid (2n = 4x = 36) promising hybrids, a sexual and an apomictic one, from crosses B. ruziziensis and B. brizantha, was evaluated. Although chromosome paired predominantly as bivalents, a few tri- and quadrivalents were recorded. Results suggest that B. brizantha and B. ruziziensis are closely related and genetic recombination is expected in hybrids. Introgression of specific target genes from B. ruziziensis into B. brizantha and vice-versa may be foreseen. However, abnormalities such as irregular chromosome segregation, chromosome stickiness and abnormal cytokinesis reported in these hybrids affect pollen fertility. More than 65% of pollen grains are sterile. Since the distinctive cytological feature of these hybrids is abnormal cytokinesis, this fact suggests that both parental genomes are unable to coordinate their activities with regard to this cytological phenomenon. Deployment of such hybrids in the process of developing varieties is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alonso, L.C. & G. Kimber, 1981. The analysis of meiosis in hybrids. II. Triploid hybrids. Can J Genet Cytol 23: 221–234.
Brown, R.C. & B.E. Lemmon, 1989. Minispindles and cytoplasmic domains in microsporogenesis in orchids. Protoplasma 148: 26–32.
Brown, R.C. & B.E. Lemmon, 1991. The cytokinetic apparatus in meiosis: Control of division plane in the absence of a preprophase band of microtubules. In: C. Lloyd (Ed.), The Cytoskeletal Basis of Plant Growth and Form, pp. 259–273. Academic Press, London.
Crane, C.F. & D.A. Sleper, 1989. A model of meiotic chromosome association in triploids. Genome 32: 82–89.
Gale, M.D. & Miller, T.E., 1987. The introduction of alien genetic variation into wheat. In: F.G.H. Lupton (Ed.), Wheat Breeding. Its Scientific Basis. Chapman & Hall, London pp. 173–210.
Juel, H., 1987. Die Kerntheilngen in den Pollenmutterzekken von Hemerocalis fulva und die bei denselben auftreden Unregelmassigkeiten. Jahrb Wiss Bot 30: 205–226.
Keller-Grein, G., B.L. Maass & J. Hanson, 1996. Natural variation in Brachiaria and existing germplasm collection. In: J.W. Miles, B.L. Maass and C.B. do Valle (Ed.) Brachiaria: Biology, Agronomy and Improvement. CIAT/Embrapa, pp. 17–42.
Mendes-Bonato, A.B., M.S. Pagliarini, C.B. do Valle & M.I.O. Penteado, 2001a. Meiotic instability in invader plants of signal grass Brachiaria decumbens Stapf (Gramineae). Acta Scientiarum 23: 619–625.
Mendes-Bonato, A.B., M.S. Pagliarini, C.B. do Valle & M.I.O. Penteado, 2001b. A severe case of chromosome stickiness in pollen mother cells of Brachiaria brizantha (Hochst) Stapf (Gramineae). Cytologia 66: 287–291.
Mendes-Bonato, A.B., R.G. Junqueira Filho, M.S. Pagliarini, C.B. do Valle & M.I O. Penteado, 2002a. Unusual cytological patterns of microsporogenesis in Brachiaria decumbens: Abnormalities in spindle and defective cytokinesis causing precocious cellularization. Cell Biol Intern 26: 641–646.
Mendes-Bonato, A.B., M.S. Pagliarini, F. Forli, C.B. do Valle & M.I.O. Penteado, 2002b. Chromosome number and microsporogenesis in Brachiaria brizantha (Gramineae). Euphytica 125: 419–425.
Miles, J.W., B.L. Maass & C.B. do Valle, 1996. Brachiaria: Biology, Agronomy, and Improvement. CIAT/Embrapa, p. 288.
Miles, J.W., C.B. do Valle, I.M. Rao, V.P.B. Euclides. 2004. Brachiaria grasses. In: L. Sollenberger (Ed.), Warm-Season (C4) Grasses, pp. 745–760. ASA, CSSA, SSSA, Madison.
Renvoize, S.A., W.D. Clayton & C.H.S. Kabuye, 1996. Morphology, taxonomy, and natural distribution of Brachiaria (Trin.) Griseb. In: J.W. Miles, et al. (Eds.), Brachiaria: Biology, Agronomy, and Improvement, pp 1–15. CIAT/Embrapa, Colombia.
Rieseberg, L.H., S.J.E. Baird & K.A. Gardner, 2000. Hybridization, introgression, and linkage evolution. Plant Mol Biol 42: 205–224.
Risso-Pascotto, C., M.S. Pagliarini, C.B. do Valle & A.B. Mendes-Bonato, 2003. Chromosome number and microsporogenesis in a pentaploid accession of Brachiaria brizantha (Gramineae). Plant Breed 122: 136–140.
Risso-Pascotto, C., M.S. Pagliarini & C.B. do Valle, 2005. Multiple spindles and cellularization during microsporogenesis in an artificially induced tetraploid accession of Brachiaria ruziziensis (Gramineae). Plant Cell Rep 23: 522–527.
Tai, W., 1970. Multipolar meiosis in diploid crested wheat-grass. Agropirum cristatum. Am J Bot 57: 1160–1169.
do Valle, C.B. & Y.H. Savidan, 1996. Genetics, cytogenetics, and reproductive biology of Brachiaria. In: J.W. Miles, B.L. Maass & C.B. do Valle (Eds.), Brachiaria: Biology, Agronomy, and Improvement, pp. 147–163. CIAT/Embrapa, Colombia.
Zwierzykowski, Z., A.J. Lukaszewski, B. Naganowska & A. Lesniewska, 1999. The pattern of homeologous recombination in triploid hybrids of Lolium multiflorum with Festuca pratensis. Genome 42: 720–726.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Risso-Pascotto, C., Pagliarini, M.S. & do Valle, C.B. Meiotic behavior in interspecific hybrids between Brachiaria ruziziensis and Brachiaria brizantha (Poaceae). Euphytica 145, 155–159 (2005). https://doi.org/10.1007/s10681-005-0893-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10681-005-0893-z