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The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast

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Abstract

With the aim of using Mytilus galloprovincialis acetylcholinesterase (AChE) and glutathione S-transferases (GST) activities as biomarkers of environmental contamination in risk assessment studies along the northwest Portuguese coast, the objective of this study was to provide background information related to: (1) baseline values of these enzymatic activities both in reference and contaminated areas and their responsiveness as indicators of exposure to environmental contaminants; (2) the importance of seasonal variations of such biomarkers in M. galloprovincialis along this area. For this, the activity of these enzymes was seasonally determined in mussels collected from four sites, including a reference and three stations with different contamination sources along the referred area. Statistically significant differences on both enzymatic activities were found among the four sampling stations, at the four sampling periods. In comparison to the reference station, lower AChE and higher GST activity values were found in mussels collected in stations potentially contaminated by pesticides and domestic/industrial effluents and in mussels collected in the vicinity of an oil refinery and an industrial/mercantile harbour, respectively. The results obtained in this work highlighted the potential suitability of these biomarkers to be used as components of environmental monitoring programs in risk assessment studies along the northwest Portuguese coast. Since a seasonal variation in both enzymatic activities was found, the possible implications of such variability in the use of these enzymes as environmental biomarkers are also discussed.

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Authors and Affiliations

  1. ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia, Universidade do Porto, Lg. Prof. Abel Salazar, 2, Porto, Portugal

    S. M. Moreira

  2. CIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Grupo de Ecotoxicologia, Rua dos Bragas 289, Porto, Portugal

    L. Guilhermino

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  1. S. M. Moreira
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  2. L. Guilhermino
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Correspondence to S. M. Moreira.

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Moreira, S.M., Guilhermino, L. The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast. Environ Monit Assess 105, 309–325 (2005). https://doi.org/10.1007/s10661-005-3854-z

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