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Evaluation of biocontrol potential of Achromobacter xylosoxidans against Fusarium wilt of melon

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Abstract

To investigate the potential of biological control measures against soil-borne plant pathogens, this study evaluated the in vitro and in vivo antifungal activity of endophytic bacteria, Achromobacter xyloxidans, against isolates of Fusarium oxysporum and Fusarium solani responsible of Fusarium wilt of melon (Cucumis melo) in Tunisia. Twenty-one Fusarium isolates were recovered and identified from diseased tissues of different melongrowing regions. The bacterial isolate was recovered and identified from contaminated Petri plates of the Fusarium phytopathogenic isolate. In vitro assay, the results showed positive effects of A. xylosoxidans by reducing significantly 80% of pathogen mycelial growth compared to the control. Furthermore, under greenhouse conditions, A. xylosoxidans significantly reduced 60% of disease severity on melon plants inoculated with Fusarium isolates. According to our results, A. xylosoxidans could be used as a promising and potentially Fusarium disease bio-control agent.

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Acknowledgments

The authors would like to thank Christie Nielsen Chaar for assistance with the English language editing.

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Authors and Affiliations

  1. Département de Santé végétale et environnement, Institut National Agronomique de Tunisie, Université de Carthage, 43 Avenue Charles Nicolle, 1082, Tunis-Mahrajène, Tunisia

    S. Dhaouadi, A. Mougou-Hamdane & B. Nasraoui

  2. Departement of Phytopathologie, INRAT, Rue Hédi Karray, 2049, Ariana, Tunisie

    W. Rouissi

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  1. S. Dhaouadi
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  2. W. Rouissi
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  3. A. Mougou-Hamdane
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  4. B. Nasraoui
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Correspondence to S. Dhaouadi.

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Funding: This study and financial support for attending symposia were funded by the laboratory of plant protection at the National Agronomic Institute of Tunisia.

Conflict of interest: The authors declare that they have no conflict of interest.

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Ethical approval: All procedures performed in this study involving human participants were in accordance with the ethical standards of the National Agronomic Institute of Tunisia.

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This chapter does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Dhaouadi, S., Rouissi, W., Mougou-Hamdane, A. et al. Evaluation of biocontrol potential of Achromobacter xylosoxidans against Fusarium wilt of melon. Eur J Plant Pathol 154, 179–188 (2019). https://doi.org/10.1007/s10658-018-01646-2

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