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Effects of elicitors on trichothecene accumulation and Tri genes expression in potato tubers inoculated with Fusarium sulphureum

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An Erratum to this article was published on 12 May 2017

Abstract

Postharvest losses due to pathogens are a major concern in agriculture and therefore new strategies to reduce these losses while making sure that the treated products are safe for the consumer, are of paramount importance. Chemical fungicides treatment is not only unsafe but also leads to pathogen developing resistance. Therefore, products that can reduce the development of dry rot of potato and mycotoxin accumulation, and be safe are needed. A better understanding of the induction of trichothecene biosynthesis is essential to reduce trichothecene production. The effects of three elicitors such as β-amino butyric acid (BABA), sodium silicate and chitosan, on the suppression of lesion development, trichothecene accumulation, and expression of Tri genes were assessed in potato tubers inoculated with Fusarium sulphureum. The results showed that lesion diameters were significantly reduced after treating with BABA at 100 mM for 3 d, sodium silicate at 100 mM for 2 d and chitosan at 0.50% for 3 d. Tri gene expressions were significantly down-regulated in inoculated tubers after elicitor treatments, and trichothecene accumulation were also suppressed. Meanwhile, the levels of trichothecene accumulation and Tri genes expression showed cumulative changes with the incubation time, extending after elicitor treatments. In addition, elicitor applications reduced more for type A trichothecene (T-2, DAS) than type B trichothecene (3ADON, Fus-X). It is possible that elicitors triggered downstream resistance genes to produce resistance related metabolites that suppressed the biomass of F. sulphureum, resulting in reduced Tri gene expressions and trichothecene accumulation.

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References

  • Bao, G. H., Bi, Y., Li, Y. C., Wu, J. T., Kou, Z. H., Ge, Y. H., Wang, Y., & Wang, D. (2013). Effect of curing duration on incidence and quality of potato tuber during storage at low temperature. Science and Technology of Food Industry, 11, 330–334 (in Chinese).

    Google Scholar 

  • Ben-Shalom, N., Ardi, R., Pinto, R., Aki, C., & Fallik, E. (2003). Controlling gray mould caused by Botrytis cinerea in cucumber plants by means of chitosan. Crop Protection, 22, 285–290.

    Article  CAS  Google Scholar 

  • Bi, Y., Tian, S. P., Zhao, J., & Ge, Y. H. (2005). Harpin induces local and systemic resistance against Trichothecium roseum in harvest Hami melons. Postharvest Biology and Technology, 38, 183–187.

    Article  Google Scholar 

  • Bojanowski, A., Avis, T. J., Pelletier, S., & Tweddell, R. J. (2013). Management of potato dry rot. Postharvest Biology and Technology, 84, 99–109.

  • Brown, D. W., Dyer, R. B., McCormick, S. P., Kendra, D. F., & Plattner, R. D. (2004). Functional demarcation of the Fusarium core trichothecene gene cluster. Fungal Genetics and Biology, 41, 454–462.

    Article  CAS  PubMed  Google Scholar 

  • Cohen, Y. (1994). 3-aminobutyric acid induces systemic resistance against Peronospore tabacina. Physiological and Molecular Plant Pathology, 44, 273–288.

    Article  CAS  Google Scholar 

  • Delgado, J. A., Schwarz, P. B., Gillespie, J., Rivera-Varas, V. V., & Secor, G. A. (2010). Trichothecene mycotoxins associated with potato dry rot caused by Fusarium sambucinum. Phytopathology, 100, 290–296.

    Article  PubMed  Google Scholar 

  • Dov, P., & Gullino, M. L. (2010). Postharvest pathology (pp. 31–34). Dordrecht, Heidelberg, London, New York: Springer. doi:10.1007/978-1-4020-8930-5.

    Google Scholar 

  • El-Ghaouth, A., Arul, J., Grenier, J., & Asselin, A. (1992). Antifungal activity of chitosan on two postharvest pathogens of strawberry fruits. Phytopathology, 82, 398–402.

    Article  CAS  Google Scholar 

  • Elsharkawy, M. M., Hase, T., Shimizu, M., & Hyakumachi, M. (2015). Suppressive effects of a polymer sodium silicate solution on powdery mildew and root rot diseases of miniature rose. African Journal of Biotechnology, 14, 2917–2927.

    Article  CAS  Google Scholar 

  • Epstein, E. (1999). Silicon. Annual Review of Plant Physiology and Molecular Biology, 50, 641–664.

    Article  CAS  Google Scholar 

  • He, J., Zhou, T., Young, J. C., Boland, G. J., & Scott, P. M. (2010). Chemical and biological transformations for detoxification of trichothecene mycotoxins in human and animal food chains: a review. Trends in Food Science and Technology, 21, 67–76.

    Article  CAS  Google Scholar 

  • Heller, J., & Tudzynski, P. (2011). Reactive oxygen species in phytopathogenic fungi: signaling, development, and disease. Annual Review of Phytopathology, 49, 369–390.

    Article  CAS  PubMed  Google Scholar 

  • Jiao, F., Kawakami, A., & Nakajima, T. (2008). Effect of carbon sources on trichothecene production and Tri gene expression by fusarium graminearum in liquid culture. FEMS Microbiology Letters, 285, 212–219.

    Article  CAS  PubMed  Google Scholar 

  • Kimura, M., Kaneko, I., Komiyama, M., Takatsuki, A., Koshino, H., Yoneyama, K., & Yamaguchi, I. (1998). Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins. The Journal of Biological Chemistry, 273, 1654–1661.

    Article  CAS  PubMed  Google Scholar 

  • Kimura, M., Tokai, T., O’Donnell, K., Ward, T. J., Fujimura, M., Hamamoto, H., Shibata, T., & Yamaguchi, I. (2003). The trichothecene biosynthesis gene cluster of fusarium graminearum F15 contains a limited number of essential pathway genes and expressed non-essential genes. FEBS Letter, 539, 105–110.

    Article  CAS  Google Scholar 

  • Kováčik, J., Klejdus, B., & Bačkor, M. (2009). Nitric oxide signals ROS scavenger-mediated enhancement of PAL activity in nitrogen-deficient Matricaria chamomilla roots: side effects of scavengers. Free Radical Biology and Medicine, 46, 1686–1693.

    Article  PubMed  Google Scholar 

  • Król, P., Igielski, R., Pollmann, S., & Kępczyńska, E. (2015). Priming of seeds with methyl jasmonate induced resistance to hemi-biotroph fusarium oxysporum f.Sp. lycopersici in tomato via 12-oxo-phytodienoic acid, salicylic acid, and flavonol accumulation. Journal of Plant Physiology, 179, 122–132.

    Article  PubMed  Google Scholar 

  • Kushalappa, A. C., Yogendra, K. N., & Karre, S. (2016). Plant innate immune response: qualitative and quantitative resistance. Critical Reviews in Plant Sciences, 35, 38–55.

    Article  CAS  Google Scholar 

  • Lee, T., Han, Y. K., Kim, K. H., Yun, S. H., & Lee, Y. W. (2002). Tri13 and Tri7 determine deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae. Applied and Environmental Microbiology., 68, 2148–2154.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li, Y. C., Bi, Y., Ge, Y. H., Sun, X. J., & Wang, Y. (2009). Antifungal activity of sodium silicate on Fusarium sulphureum and its effect on dry rot of potato tubers. Journal of Food Science, 74, 213–218.

    Article  CAS  Google Scholar 

  • Li, Y. C., Yang, Z. M., Bi, Y., Zhang, J. L., & Wang, D. (2012). Antifungal effect of borates against Fusarium sulphureum on potato tubers and it possible mechanisms of action. Postharvest Biology and Technology, 74, 55–61.

    Article  CAS  Google Scholar 

  • Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2∆∆CT method. Methods, 25, 402–408.

    Article  CAS  PubMed  Google Scholar 

  • Lulai, E. C., & Neubauer, J. D. (2014). Wound-induced suberization genes are differentially expressed, spatially and temporally, during closing layer and wound periderm formation. Postharvest Biology and Technology, 90, 24–33.

    Article  CAS  Google Scholar 

  • Ma, F. R., Zhang, Y. C., Tian, F., & Zhang, F. J. (2011). Effect of different fertilizer amount and density on potato yield in alpine-cold region. Journal of Changjiang Vegetables, 2, 55–58 (in Chinese).

    Google Scholar 

  • McCormick, S. P., Stanley, A. M., Stover, N. A., & Alexander, N. J. (2011). Trichothecenes: from simple to complex mycotoxins. Toxins, 3, 802–814.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Merhej, J. A., Boutigny, L., Pinson-Gadais, L., Richard-Forget, F., & Barreau, C. (2010). Acidic pH as a determinant of TRI gene expression and trichothecene B biosynthesis in Fusarium graminearum. Food Additives and Contaminants, 27, 710–717.

    Article  CAS  PubMed  Google Scholar 

  • Nicholson, P., Simpson, D. R., Wilson, A. H., Chandler, E., & Thomsett, M. (2004). Detection and differentiation of trichothecene and Enniatin-producing Fusarium species on small-grain cereals. European Journal of Plant Pathology, 110, 503–514.

    Article  CAS  Google Scholar 

  • Nishiuchi, T., Masuda, D., Nakashita, H., & Ichimura, K. (2006). Fusarium phytotoxin trichothecenes have an elicitor-like activity in Arabidopsis thaliana, but the activity differed significantly among their molecular species. Molecular Plant-Microbe Interactions, 19, 512–520.

    Article  CAS  PubMed  Google Scholar 

  • Niu, L. L., Bi, Y., Bai, X. D., Zhang, S. G., Xue, H. L., Li, Y. C., Wang, Y., & Calderón-Urrea, A. (2016). Damage to Trichothecium roseum caused by sodium silicate is independent from pH. Canadian Journal of Microbiology, 62, 161–172.

    Article  CAS  PubMed  Google Scholar 

  • Olivieri, F. P., Lobato, M. C., Gonzalez Altamiranda, E., Daleo, G. R., Huarte, M., Guevara, M. G., & Andreu, A. B. (2009). BABA effects on the behaviour of potato cultivars infected by Phytophthora infestans and Fusarium solani. European Journal of Plant Pathology, 132, 47–56.

    Article  Google Scholar 

  • Ponts, N., Pinson-Gadais, L., Barreau, C., Richard-Forget, F., & Ouellet, T. (2007). Exogenous H2O2 and catalase treatments interfere with Tri genes expression in liquid cultures of fusarium graminearum. FEBS Letters, 581, 443–447.

    Article  CAS  PubMed  Google Scholar 

  • Porat, R., Vinokur, V., Weiss, B., Cohen, L., Daus, A., Goldschmidt, E. E., & Droby, S. (2003). Induction of resistance to Penicillium digitatum in grapefruit by β-aminobutyric acid. European Journal of Plant Pathology, 109, 901–907.

    Article  CAS  Google Scholar 

  • Reuveni, M., Zahavi, T., & Cohen, Y. (2001). Controlling downy mildew (Plasmopara viticola) in field-grown grapevine with β-aminobutyric acid (BABA). Phytoparasitica, 29, 125–133.

    Article  CAS  Google Scholar 

  • Romanazzi, G., Nigro, F., Ippolito, A., DiVenere, D., & Salerno, M. (2002). Effects of pre- and postharvest chitosan treatments to control storage grey mold of table grapes. Journal of Food Science, 67, 1862–1867.

    Article  CAS  Google Scholar 

  • Romanazzi, G., Sanzani, S. M., Bi, Y., Tian, S. P., Martínez, P. G., & Alkan, N. (2016a). Induced resistance to control postharvest decay of fruit and vegetables. Postharvest Biology and Technology, 122, 82–94.

    Article  CAS  Google Scholar 

  • Romanazzi, G., Smilanick, J. L., Feliziani, E., & Droby, S. (2016b). Integrated management of postharvest gray mold on fruit crops. Postharvest Biology and Technology, 113, 69–76.

    Article  CAS  Google Scholar 

  • Seong, K. Y., Pasquali, M., Zhou, X., Song, J., Hilburn, K., McCormick, S., Dong, Y., Xu, J. R., & Kistler, H. C. (2009). Global gene regulation by Fusarium transcription factors Tri6 and Tri10 reveals adaptations for toxin biosynthesis. Molecular Microbiology, 72, 354–367.

    Article  CAS  PubMed  Google Scholar 

  • Stefańczyk, E., Sobkowiak, S., Brylińska, M., & Śliwka, J. (2016). Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland. European Journal of Plant Pathology. doi:10.1007/s10658-016-0875-0.

    Google Scholar 

  • Sun, X. J., Bi, Y., Li, Y. C., Han, R. F., & Ge, Y. H. (2008). Postharvest chitosan treatment induces resistance in potato against Fusarium sulphureum. Agricultural Sciences in China, 7, 615–621.

    Article  CAS  Google Scholar 

  • Takahashi-Ando, N., Ochiai, N., Tokai, T., Ohsato, S., Nishiuchi, T., Yoshida, M., Fujimura, M., & Kimura, M. (2008). A screening system for inhibitors of trichothecene biosynthesis: hydroxylation of trichodiene as a target. Biotechnology Letter, 30, 1055–1059.

    Article  CAS  Google Scholar 

  • Tang, Y. M., Xue, H. L., Bi, Y., Li, Y. C., Wang, Y., Zhao, Y., & Shen, K. P. (2015). A method for analysis of T-2 toxin and neosolaniol by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry in apple fruits inoculated with Trichothecium roseum. Food Additives & Contaminants- Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 32, 480–487.

    CAS  Google Scholar 

  • Thomas, M. H., & Frank, V. (1986). Purification and characterization of the sesquiterpene cyclase trichodiene synthetase from Fusarium sporotrichioides. Archives of Biochemistry and Biophysics, 251, 756–761.

    Article  Google Scholar 

  • Torres, M. A., Jones, J. D. G., & Dangl, J. L. (2005). Pathogen-induced, NADPH oxidase-derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana. Nature. Genetic, 37, 1130–1134.

    CAS  Google Scholar 

  • Tripathi, P., & Dubey, N. K. (2004). Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest Biology and Technology, 32, 235–245.

    Article  Google Scholar 

  • Wang, Y., Duan, Y. B., & Zhou, M. G. (2016). Baseline sensitivity and efficacy of fluazinam in controlling Sclerotinia stem rot of rapeseed. European Journal of Plant Pathology, 144, 337–343.

    Article  CAS  Google Scholar 

  • Xue, H. L., Bi, Y., Wei, J. M., Tang, Y. M., Zhao, Y., & Wang, Y. (2013). New method for the simultaneous analysis of types a and B trichothecenes by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry in potato tubers inoculated with Fusarium sulphureum. Journal of Agricultural and Food Chemistry, 61, 9333–9338.

    Article  CAS  PubMed  Google Scholar 

  • Zhao, Y. H., Lin, N. N., Guo, L., Chen, J., Liu, W. H., & Xie, J. W. (2012). Simultaneous determination of T-2 toxin and its metabolites in rat plasma using solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry. Chinese Journal of Analytical Chemistry, 12, 1852–1858.

    Google Scholar 

  • Zhao, Y., Xue, H. L., Bi, Y., Tang, Y. M., & Shen, K. P. (2015). T-2 toxin at low concentration activated phenylpropanoid pathway in potato tubers. Food Science, 36, 232–235 (in Chinese).

    Google Scholar 

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Acknowledgements

This work was funded by the Ministry of Science and Technology of China (2016YFD04009-05), the Natural Science Foundation of China (31560475) and the National High Technology Research and Development Program of China (863 Program, No. 2012AA101607) and the program for Fuxi talents in GAU (FXRC20130105) and the Program China Scholarship Council.

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Correspondence to Bi Yang.

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An erratum to this article is available at http://dx.doi.org/10.1007/s10658-017-1242-5.

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Hua-Li, X., Yang, B., Yuan-Yuan, Z. et al. Effects of elicitors on trichothecene accumulation and Tri genes expression in potato tubers inoculated with Fusarium sulphureum . Eur J Plant Pathol 148, 673–685 (2017). https://doi.org/10.1007/s10658-016-1125-1

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