Skip to main content

Advertisement

SpringerLink
Log in

Fusarium species associated with date palm in Saudi Arabia

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Fusarium is one of the most destructive fungal genera whose members cause many diseases on plants, animals, and humans. Moreover, many Fusarium species secrete mycotoxins (e.g. trichothecenes and fumonisins) that are toxic to humans and animals. Fusarium isolates from date palm trees showing disease symptoms, e.g. chlorosis, necrosis and whitening, were collected from seven regions across Saudi Arabia. After single-sporing, the fungal strains were morphologically characterized. To confirm the identity of morphologically characterized Fusarium strains, three nuclear loci, two partial genes of translation elongation factor 1 α (tef1α) and β-tubulin (tub2), and the rDNA-ITS region, were amplified and sequenced. Of the 70 Fusarium strains, 70 % were identified as F. proliferatum that were recovered from six regions across Saudi Arabia. Fusarium solani (13 %), as well as one strain each of the following species: F. brachygibbosum, F. oxysporum, and F. verticillioides were also recovered. In addition, five Fusarium-like strains were recognized as Sarocladium kiliense by DNA-based data. The preliminary in vitro pathogenicity results showed that F. proliferatum had the highest colonization abilities on date palm leaflets, followed by F. solani. Although F. oxysporum f. sp. albedinis is the most serious date palm pathogen, F. proliferatum and F. solani are becoming serious pathogens and efforts should be made to restrict and control them. In addition, the potential toxin risks of strains belonging to F. proliferatum should be evaluated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Abdalla, M. Y., Al-Rokibah, A., Moretti, A., & Mulé, G. (2000). Pathogenicity of toxigenic Fusarium proliferatum from date palm in Saudi Arabia. Plant Disease, 84(3), 321–324.

    Article  Google Scholar 

  • Al-Abdely, H. M. (2004). Management of rare fungal infections. Current Opinion in Infectious Diseases, 17(6), 527–532.

    Article  PubMed  Google Scholar 

  • Al-Julaifi, M. Z., & Al-Falih, A. M. (2001). Detection of trichothecenes in animal feeds and food stuffs during the years 1997 to 2000 in Saudi Arabia. Journal of Food Protection, 64(10), 1603–1606.

    Article  CAS  PubMed  Google Scholar 

  • Alkahtani, M., EL-Naggar, M. A., Omer, S. A., Abdel-Kareem, E. M., & Ammar, M. F. (2011). Effect of toxic Fusarium moniliforme on some biochemical component of some date palm cultivars. American Journal of Food Technology, 6(9), 730–741.

    Article  CAS  Google Scholar 

  • Al-Sadi, A. M., Al-Jabri, A. H., Al-Mazroui, S. S., & Al-Mahmooli, I. H. (2012). Characterization and pathogenicity of fungi and oomycetes associated with root diseases of date palms in Oman. Crop Protection, 37, 1–6.

    Article  Google Scholar 

  • Al-Sadi, A. M., Al-Masoodi, R. S., Al-Ismaili, M., & Al-Mahmooli, I. H. (2015). Population structure and development of resistance to Hymexazol among Fusarium solani populations from date palm, citrus and cucumber. Journal of Phytopathology, 163(11–12), 947–955.

    Article  CAS  Google Scholar 

  • Al-Yasiri, N. A., Saad, A. R., Nasser, S. A., & Zaid K. M. (2010). The relationship between the fungus Fusarium solani and some pathological phenomena on date palm trees and the effectiveness of some systemic fungicide for their control. Proceeding of the Fourth International Date Palm Conference, March 15–17. 505–514.

  • Armengol, J., Moretti, A., Perrone, G., Vicent, A., Bengoechea, J. A., & García-Jiménez, J. (2005). Identification, incidence and characterization of Fusarium proliferatum on ornamental palms in Spain. European Journal of Plant Pathology, 112(2), 123–131.

    Article  Google Scholar 

  • Betts, M. J., & Russell, R. B. (2003). Amino acid properties and consequences of substitutions. In M. R. Barnes & I. C. Gray (Eds.), Bioinformatics for geneticists (pp. 289–316). Chichester: Wiley.

    Chapter  Google Scholar 

  • Bokhary, H. A. (2006). Seed-borne fungi of date-palm, L. from Saudi Arabia. Saudi Journal of Biological Sciences, 17(4), 327–329.

    Article  Google Scholar 

  • Cohen, Y., Freeman, S., Zveibil, A., Ben Zvi, R., Nakache, Y., Biton, S., & Soroker, V. (2010). Reevaluation of factors affecting bunch drop in date palm. Hortscience, 45(6), 887–893.

    Google Scholar 

  • Desjardins, A. E. (2006). Fusarium mycotoxins: chemistry, genetics and biology. St. Paul (MN): APS Press.

    Google Scholar 

  • Djerbi, M. (1982). Bayoudh disease in North Africa, history, distribution, diagnosis and control. Date Palm Jounal, 1, 153–197.

    Google Scholar 

  • Doval, S. L., Kaul, C. K., & Mathur, B. P. (1976). Note on control of mango malformation. Indian Journal of Agricultural Sciences, 46(11), 545–546.

    Google Scholar 

  • Elliott, M. L., Des Jardin, E. A., O’Donnell, K., Geiser, D. M., Harrison, N. A., & Broschat, T. K. (2010). Fusarium oxysporum f. Sp. palmarum, a novel forma specialis causing a lethal disease of Syagrus romanzoffiana and Washingtonia robusta in Florida. Plant Disease, 94(1), 31–38.

    Article  CAS  Google Scholar 

  • Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4), 783–791.

    Article  Google Scholar 

  • Flood, J. (2006). A review of fusarium wilt of oil palm caused by Fusarium oxysporum f. Sp. elaeidis. Phytopathology, 96(6), 660–662.

    Article  PubMed  Google Scholar 

  • Gashgari, R. M., Shebany, Y. M., & Gherbawy, Y. A. (2010). Molecular characterization of mycobiota and aflatoxin contamination of retail wheat flours from Jeddah markets. Foodborne Pathogens and Disease, 7(9), 1047–1054.

    Article  CAS  PubMed  Google Scholar 

  • Gelderblom, W. C. A., Marasas, W. F. O., Lebepe-Mazur, S., Swanevelder, S., & Abel, S. (2008). Cancer initiating properties of fumonisin B1 in a short-term rat liver carcinogenesis assay. Toxicology, 250(2–3), 89–95.

    Article  CAS  PubMed  Google Scholar 

  • Gillespie, D. R., & Menzies, J. G. (1993). Fungus gnats vector Fusarium oxysporum f. Sp. radicis-lycopersici. Annals of Applied Biology, 123(3), 539–544.

    Article  Google Scholar 

  • Hameed, M. A. (2012). Inflorescence rot disease of date palm caused by Fusarium proliferatum in southern Iraq. African Journal of Biotechnology, 11(35), 8616–8612.

    Article  CAS  Google Scholar 

  • Hartmann, N., Erbs, M., Forrer, H. R., Vogelgsang, S., Wettstein, F. E., Schwarzenbach, R. P., & Bucheli, T. D. (2008). Occurrence of zearalenone on Fusarium graminearum infected wheat and maize fields in crop organs, soil and drainage water. Environmental Science and Technology, 42(15), 5455–5460.

    Article  CAS  PubMed  Google Scholar 

  • Hernández-Hernández, J., Espino, A., Rodríguez-Rodríguez, J. M., Pérez-Sierra, A., León, M., Abad-Campos, P., & Armengol, J. (2010). Survey of diseases caused by Fusarium spp. on palm trees in the Canary Islands. Phytopathologia Mediterranea, 49(1), 84–88.

    Google Scholar 

  • Jarvis, W. R., Shipp, J. L., & Gardiner, R. B. (1993). Transmission of Pythium aphanidermatum to greenhouse cucumber by the fungus gnat Bradysia impatiens (Diptera: Sciaridae). Annals of Applied Biology, 122(1), 23–29.

    Article  Google Scholar 

  • Khairi, M. M. A., Elhassan, M. I., & Bashab, F. A. (2010). The status of date palm cultivation and date production in Sudan. Acta Horticulturae, 882, 37–42.

    Article  Google Scholar 

  • Khudhair, M. W., Aboud, H. M., Khalaf, M. Z., & Shbar, A. K. (2014). Oryctes elegans, a vector of Fusarium proliferatum causing wilt disease symptoms of date palm. International Journal of Phytoremediation, 3(2), 67–72.

    Google Scholar 

  • Klumpp, L. M., Mackey, A. T., Farrell, C. M., Rosenberg, J. M., & Gilbert, S. P. (2003). A kinesin switch I arginine to lysine mutation rescues microtubule function. The Journal of Biological Chemistry, 278(40), 39059–39067.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kvas, M., Marasas, W. F. O., Wingfield, B. D., Wingfield, M. J., & Steenkamp, E. T. (2009). Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex. Fungal Diversity, 34, 1–21.

    Google Scholar 

  • Lemmer, E. R., Vessey, C. J., Gelderblom, W. C. A., Shephard, E. G., Van Schalkwyk, D. J., Rochelle, D. J., Van Wijk, A., Marasas, W. F. O., Kirsch, R. E., & Hall, P. M. (2004). Fumonisin B1-induced hepatocellular and cholangiocellular tumors in male Fischer 344 rats: potentiating effects of 2-acetylaminofluorene on oval cell proliferation and neoplastic development in a discontinued feeding study. Carcinogenesis, 25(7), 1–8.

    Article  Google Scholar 

  • Leslie, J. F., & Summerell, B. A. (2006). The fusarium laboratory manual. Ames (IA): Blackwell.

    Book  Google Scholar 

  • Maitlo, W. A., Markhand, G. S., Abul-Soad, A. A., Lodhi, A. M., & Jatoi, M. A. (2014). Fungi associated with sudden decline disease of date palm (Phoenix dactylifera L.) and its incidence at Khairpur, Pakistan. Pakistan Journal of Phytopathology, 26(1), 67–73.

    Google Scholar 

  • Mandeel, Q., Ayub, N., & Gul, J. (2005). Survey of Fusarium species in an environment of Bahrain. VI. Biodiversity of the genus Fusarium in root-soil ecosystem of halophytic date palm (Phoenix dactylifera) community. Cryptogamie Mycologie, 26(4), 365–404.

    Google Scholar 

  • Mansoori, B. (2012). Fusarium proliferatum induces gum in xylem vessels as the cause of date bunch fading in Iran. Journal of Agricultural Science and Technology, 14(5), 1133–1140.

    Google Scholar 

  • Mansoori, B., & Kord, M. (2006). Yellow death: a disease of date palm in Iran caused by Fusarium solani. Journal of Phytopathology, 154(2), 125–127.

    Article  Google Scholar 

  • Marasas, W. F. O. (2001). Discovery and occurrence of the fumonisins: a historical perspective. Environmental Health Perspectives, 109(2), 239–243.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Meldrum, R. A., Daly, A. M., Tran-Nguyen, L. T. T., & Aitken, E. A. B. (2013). Are banana weevil borers a vector in spreading Fusarium oxysporum f. Sp. cubense tropical race 4 in banana plantations? Australasian Plant Pathology, 42(5), 543–549.

    Article  CAS  Google Scholar 

  • Munoz, A., & Wang, S. (2011). Detection of Fusarium oxysporum f. Sp canariensis and F. proliferatum from palms in southern Nevada. Phytopathology, 101, S125–S125.

    Google Scholar 

  • Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19), 4321–4325.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Musa, M. O., Al Eisa, A., Halim, M., Sahovic, E., Gyger, M., Chaudhri, N., Al Mohareb, F., Seth, P., Aslam, M., & Aljurf, M. (2000). The spectrum of Fusarium infection in immunocompromised patients with haematological malignancies and in non-immunocompromised patients: a single institution experience over 10 years. British Journal of Haematology, 108(3), 544–548.

    Article  CAS  PubMed  Google Scholar 

  • O’Donnell, K., & Cigelnik, E. (1997). Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution, 7(1), 103–116.

    Article  PubMed  Google Scholar 

  • O’Donnell, K., Cigelnik, E., & Nirenberg, H. I. (1998a). Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia, 90(3), 465–493.

    Article  Google Scholar 

  • O’Donnell, K., Kistler, H. C., Cigelnik, E., & Ploetz, R. C. (1998b). Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences, 95(5), 2044–2049.

    Article  Google Scholar 

  • O’Donnell, K., Nirenberg, H. I., Aoki, T., & Cigelnik, E. (2000). A multigene phylogeny of the Gibberella fujikuroi species complex: detection of additionally phylogenetically distinct species. Mycoscience, 41(1), 61–78.

    Article  Google Scholar 

  • Polizzi, G., & Vitale, A. (2003). First report of fusarium blight on majesty palm caused by Fusarium proliferatum in Italy. Plant Disease, 87(9), 1149.

    Article  Google Scholar 

  • Prouillac, C., Videmann, B., Mazallon, M., & Lecoeur, S. (2009). Induction of cells differentiation and ABC transporters expression by a myco-estrogen, zearalenone, in human choriocarcinoma cell line (BeWo). Toxicology, 263(2–3), 100–107.

    Article  CAS  PubMed  Google Scholar 

  • Saleh, A. A., El_Komy, M. H., Eranthodi, A., Hamoud, A. S., & Molan, Y. Y. (2015). Variation in a molecular marker for resistance of Saudi date palm germplasm to Fusarium oxysporum f. Sp. albedinis the causal agent of Bayoud disease. European Journal of Plant Pathology, 143(3), 507–514.

    Article  CAS  Google Scholar 

  • Salgado-Neto, G., Vaz, M., Guedes, J., Muniz, M., & Blume, E. (2016). Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil. Ciência Rural, 46(6), 943–949.

    Article  Google Scholar 

  • Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Seifert, K. A., Aoki, T., Baayen, R. P., Brayford, D., Burgess, L. W., Chulze, S., Gams, W., Geiser, D., de Gruyter, J., Leslie, J. F., Logrieco, A., Marasas, W. F. O., Nirenberg, H. I., O’Donnell, K., Rheeder, J., Samuels, G. J., Summerell, B. A., Thrane, U., & Waalwijk, C. (2003). The name Fusarium moniliforme should no longer be used. Mycological Research, 107(6), 643–644.

    Article  Google Scholar 

  • Stanghellini, M. E., Rasmussen, S. L., & Kim, D. H. (1999). Aerial transmission of Thielaviopsis basicola, a pathogen of corn-salad, by adult shore flies. Phytopathology, 89(6), 476–479.

    Article  CAS  PubMed  Google Scholar 

  • Stępień, L., Koczyk, G., & Waśkiewicz, A. (2011). Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species. Journal of Applied Genetics, 2, 487–496.

    Google Scholar 

  • Summerbell, R. C., Gueidan, C., Schroers, H.-J., de Hoog, G. S., Starink, M., Arocha Rosete, Y., Guarro, J., & Scott, J. A. (2011). Acremonium phylogenetic overview and revision of Gliomastix, Sarocladium, and Trichothecium. Studies in Mycology, 68, 139–162.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Swofford, D. L. (2002). PAUP*, Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sunderland: Sinauer Associates.

    Google Scholar 

  • White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplified and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: a guide to methods and applications (pp. 315–322). San Diego: Academic Press.

    Google Scholar 

  • Yeh, Y., & Kirschner, R. (2014). Sarocladium spinificis, a new endophytic species from the coastal grass Spinifex littoreus in Taiwan. Botanical Studies, 55, 25.

    Article  Google Scholar 

  • Zaid, A., De Wet, P. F., Djerbi, M., & Oihabi, A. (2002). Chapter XII diseases and pests of date palm. In A. Zaid (Ed.), Date palm cultivation (pp. 223–287). Rome: Food and Agriculture Organization of the United Nations.

    Google Scholar 

Download references

Acknowledgment

This Project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (11-AGR1475-02).

Author information

Authors and Affiliations

  1. Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, P. O. Box 2460, Riyadh, 11451, Saudi Arabia

    Amgad A. Saleh, Anwar H. Sharafaddin, Mahmoud H. El_Komy, Yasser E. Ibrahim, Younis K. Hamad & Younis Y. Molan

  2. Agriculture Research Center, Agricultural Genetic Engineering Research Institute, Giza, Egypt

    Amgad A. Saleh

  3. Agriculture Research Center, Plant Pathology Institute, Giza, Egypt

    Mahmoud H. El_Komy & Yasser E. Ibrahim

  4. Plant Pathology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

    Younis K. Hamad

Authors
  1. Amgad A. Saleh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anwar H. Sharafaddin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mahmoud H. El_Komy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yasser E. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Younis K. Hamad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Younis Y. Molan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amgad A. Saleh.

Electronic supplementary material

ESM 1

(DOCX 34 kb)

ESM 2

(DOCX 386 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saleh, A.A., Sharafaddin, A.H., El_Komy, M.H. et al. Fusarium species associated with date palm in Saudi Arabia. Eur J Plant Pathol 148, 367–377 (2017). https://doi.org/10.1007/s10658-016-1095-3

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-016-1095-3

Keywords

Search

Navigation