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New sources of resistance to Septoria tritici blotch in wheat seedlings

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Abstract

The resistance of a set of Argentine wheat cultivars, differentials, and foreign lines with known genes of resistance to Zymoseptoria tritici was assessed at the seedling stage against a set of molecularly characterized Argentine fungal isolates under three experimental conditions. The experimental design was a split-split-plot with two replications. The environment conditions were the main plots, the fungal isolates were the subplots and the wheat lines with known resistance genes or used as differential lines or the Argentine commercial cultivars were the sub-subplots. Sixteen fungal isolates were inoculated on 23 wheat accessions including 12 foreign lines with known resistance genes, two differential lines, and nine Argentine cultivars that were selected on the basis of their moderate resistance to natural infection, to identify isolate-specific and partial resistances. The genotypes TE 9111 and Oasis among the foreign lines along with the Argentine cultivars Pro INTA Puntal, Klein Volcán and Buck 75 Aniversario expressed the highest number of specific-resistance interactions. Flame, Tadinia and Kavkaz among the foreign lines showed partial resistances to a higher number of fungal isolates; a comparable resistance pattern was also obtained in the Argentine cultivars Klein Sagitario, Klein Dragón, Don Ernesto and Buck Arriero. Our study demonstrated that the presence of resistance genes in several foreign lines against Septoria tritici blotch is effective against some of the Argentine fungal isolates. In addition, the Argentinean wheat cultivars that showed isolate-specific and nonspecific resistances probably carry novel resistance genes against the pathogen.

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Acknowledgments

This work was supported by grants provided by ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica) (PICT 08–14489), Universidad Nacional de La Plata (A189 and A 227) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).

We thank Fabiana Consolo and Clara Albani from FIBA (Fundación para Investigaciones Biológicas Aplicadas), Mar del Plata, Buenos Aires Province, Argentina, for their generous cooperation during our molecular testing and Dr. Donald F. Haggerty, a retired career investigator and native English speaker, edited the final version of the manuscript.

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Authors and Affiliations

  1. Cerealicultura, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, CC 31, La Plata, 1900, Argentina

    María Rosa Simón

  2. IGEVET (Instituto de Genética Veterinaria Dr. Fernando Dulout)- CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 117, CC 31, La Plata, 1900, Argentina

    Nadia S. Castillo

  3. CIDEFI (Centro de Investigaciones de Fitopatología)- CIC (Centro de Investigaciones Científicas de la Provincia de Buenos Aires), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, CC 31, La Plata, 1900, Argentina

    Cristina A. Cordo

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  1. María Rosa Simón
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  2. Nadia S. Castillo
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  3. Cristina A. Cordo
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Correspondence to María Rosa Simón or Nadia S. Castillo.

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María Rosa Simón and Nadia S. Castillo contributed equally.

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Simón, M.R., Castillo, N.S. & Cordo, C.A. New sources of resistance to Septoria tritici blotch in wheat seedlings. Eur J Plant Pathol 146, 625–635 (2016). https://doi.org/10.1007/s10658-016-0948-0

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