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Screening chilli (Capsicum spp.) germplasm against Cucumber mosaic virus and Chilli veinal mottle virus and inheritance of resistance

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Abstract

Cucumber mosaic virus (CMV) and Chilli veinal mottle virus (ChiVMV) are aphid-borne major destructive viruses affecting chilli crops. Identification of resistant sources and understanding the genetic behaviour of resistance is essential for resistance breeding. In this study, fifty Capsicum genotypes were screened for CMV and ChiVMV resistance through mechanical inoculation. Symptoms varying from chlorotic local lesions to severe leaf distortion were observed and among the genotypes eighteen immune, eight highly resistant, five resistant and two moderately resistant genotypes were identified against CMV. For ChiVMV, dark green vein banding to severe shoe string symptoms were observed and among genotypes, seventeen were immune, one highly resistant, five resistant and two moderately resistant. Ten parents were selected and crossed in diallel fashion without reciprocals to produce 45 F1 hybrids and were screened for resistance against both the viruses separately. Only one hybrid IHR 2451 x IHR 500 showed resistance against CMV and all resistant x resistant F1 hybrids i.e. IHR 4503 x IHR 2451, IHR 4503 x IHR 500, IHR 2451 x IHR 500, IHR 3849 x IHR 2451 and IHR 3849 x IHR 500 were found resistant against ChiVMV. Further the inheritance studies using advanced populations showed that the CMV resistance is polygenic recessive and ChiVMV resistance is monogenic recessive in IHR 2451 and IHR 4503.

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References

  • Ali, M., Hossain, M. Z., & Sarker, N. C. (2000). Inheritance of yellow vein mosaic virus (YVMV) tolerance in a cultivar of okra (Abelmoschus esculantus (L.) Moench). Euphytica, 111, 205–209.

    Article  Google Scholar 

  • Caranta, C., Palloix, A., Lefebvre, V., & Daubeze, A. M. (1997a). QTLs for a component of partial resistance to cucumber mosaic virus in pepper: restriction of virus installation in host cells. Theoretical and Applied Genetics, 94, 431–438.

    Article  CAS  Google Scholar 

  • Caranta, C., Lefebvre, V., & Palloix, A. (1997b). Polygenic resistance of pepper to poty viruses consists of a combination of isolate-specific and broad-spectrum quantitative trait loci. Molecular Plant-Microbe Interactions, 10, 872–878.

    Article  CAS  Google Scholar 

  • Caranta, C., Pflieger, S., Lefebvre, V., Daubeze, A. M., Thabuis, A., & Palloix, A. (2002). QTLs involved in the restriction of cucumber mosaic virus (CMV) long-distance movement in pepper. Theoretical and Applied Genetics, 104, 586–591.

    Article  CAS  PubMed  Google Scholar 

  • Chaim, B. A., Grube, R. C., Lapidot, M., Jahn, M., & Paran, I. (2001). Identification of quantitative trait loci associated with resistance to cucumber mosaic virus in Capsicum annuum. Theoretical and Applied Genetics, 102, 1213–1220.

    Article  Google Scholar 

  • Chew, B. H., & Ong, C. A. (1990). Genetics and breeding for chilli veinal mottle and cucumber mosaic virus resistances in hot pepper proceedings of the 3rd International Conference on plant Protection in the Tropics: Volume V (pp. In 55–58). Pahang: Malaysia.

    Google Scholar 

  • Duriat, A. S., Gunaeni, N., & Sulastrini, I. (1997). Further studies on screening pepper varieties for resistance to CMV strains, In: AVRDC Collaborative vegetable research in Southeast Asia proceedings of the AVNET-II final workshop, Publication No. 119–124 (pp 451). Thailand: Bangkok.

    Google Scholar 

  • Green, S. K., & Kim, J. S. (1991). Characteristics and control of viruses infecting peppers: a Literature Review, Technical Bulletin no. 18. Taiwan: AVRDC.

    Google Scholar 

  • Green, S. K., & Kim, J. S. (1994). Sources of resistance to viruses of pepper (Capsicum spp.): a catalog (pp 7–10). Taiwan: AVRDC.

    Google Scholar 

  • Grube, R. C., Blauth, J. R., Andredos, M. S. A., Caranta, C., & Jahn, M. K. (2000a). Identification and comparative mapping of a dominant potyvirus resistance gene cluster in capsicum. Theoretical and Applied Genetics, 101, 852–859.

    Article  CAS  Google Scholar 

  • Grube, R. C., Zhang, Y., Murphy, J. F., Loaiza-Figueroa, F., Lackney, V. K., Provvidenti, R., & Jahn, M. (2000b). New source of resistance to cucumber mosaic virus in Capsicum frutescens. Plant Disease, 84, 885–891.

    Article  Google Scholar 

  • Hidayat, S. H., Opriana, E., Manzila, I., & Sujiprihati, S. (2012). Occurrence of chilli veinal mottle virus (ChiVMV) in Indonesia and response of chilli germplasms to ChiVMV infection. The Journal of International Society for Southeast Asian Agricultural Sciences, 18(2), 55–61.

    Google Scholar 

  • Hobbs, H. A., Reddy, D. V. R., Rajeshwari, R., & Reddy, A. S. (1987). Use of direct antigen coating and protein A coating ELISA procedures for detection of three peanut viruses. Plant Disease, 71, 747–749.

    Article  Google Scholar 

  • Hwang, J., Li, J., Liu, W. Y., An, S. J., Cho, H., Her, N. H., Yeam, I., Kim, D., & Kang, B. C. (2009). Double mutations in eIF4E and eIFiso4E confer recessive resistance to chilli veinal mottle virus in pepper. Molecules and Cells, 27, 329–336.

    Article  CAS  PubMed  Google Scholar 

  • Kang, W. H., Hoang, N. H., Yang, H. B., Kwon, J. K., Jo, S. H., Seo, J. K., Kim, K. H., Choi, D., & Kang, B. C. (2010). Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.). Theoretical and Applied Genetics, 120, 1587–1596.

    Article  CAS  PubMed  Google Scholar 

  • Lapidot, M., Paran, I., Ben-Joseph, R., Ben-Harush, S., Pilowsky, M., Cohen, S., & Shifriss, C. (1997). Tolerance to cucumber mosaic virus in pepper: development of advanced breeding lines and evaluation of virus level. Plant Disease, 81, 185–188.

    Article  Google Scholar 

  • Lee, H. R., An, H. J., You, Y. G., Lee, J., Kim, H. J., Kang, B. C., & Harn, C. H. (2013). Development of a novel codominant molecular marker for chilli veinal mottle virus resistance in Capsicum annuum L. Euphytica, 193, 197–205.

    Article  CAS  Google Scholar 

  • Moury, B., Palloix, A., Caranta, C., Gognalons, P., Souche, S., Selassie, K. G., & Marchoux, G. (2005). Serological, molecular, and pathotype diversity of pepper veinal mottle virus and chilli veinal mottle virus. Phytopathology, 95(3), 227–232.

    Article  CAS  PubMed  Google Scholar 

  • Nono-Womdim, R., Marchoux, G., Pochard, E., Palloix, A., & Gebre-Selassie, K. (1991). Resistance of pepper lines to the movement of Cucumber mosaic virus. Journal of Phytopathology, 132, 21–32.

    Article  Google Scholar 

  • Palukaitis, P., Roossinck, M. J., Dietzgen, R. G., & Francki, R. I. B. (1992). Cucumber mosaic virus. Advances in Virus Research, 41, 281–341.

    Article  CAS  PubMed  Google Scholar 

  • Pochard, E. (1977). Methods for the study of partial resistance to cucumber mosaic virus in pepper. Capsicum 77. In Proc. 3rd EUCARPIA Meeting (pp 93–104). Avignon-Montfavet: France

  • Pochard, E., & Daubeze, A. M. (1989). Progressive construction of a polygenic resistance to cucumber mosaic virus in the pepper. In Proceedings of EUCARPIA meeting on genetics and breeding of capsicum and Eggplant (pp. 189–192). Kragujevac: Yugoslavia.

    Google Scholar 

  • Provvidenti, R., & Hampton, R. O. (1992). Sources of resistance to viruses in the potyviridae (pp 189–211). Vienna: Springer.

    Google Scholar 

  • Reddy, M. K., Sadhashiva, A. T., Reddy, K. M., Chalam, C., Deshpande, A. A., & Chandro, A. (2001). Integrated disease and pest management: leaf curl and other viruses of tomato and peppers. proceedings of the final workshop (pp 3–8). Bangkok: Thailand.

    Google Scholar 

  • Reddy, M. K., Srivastava, A., Kumar, S., Kumar, R., Chawda, N., Ebert, A. W., & Vishwakarma, M. (2014). Chilli (Capsicum annuum L.) breeding in India: an overview. SABRAO Journal of Breeding and Genetics, 46, 160–173.

  • Roossinck, M. J. (2002). Evolutionary history of cucumber mosaic virus detected by phylogenetic analyses. Journal of Virology, 76(7), 3382–3387.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shah, H., Yasmin, T., Fahim, M., Hameed, S., Irfan-Ul-Haque, Munir, M. & Khanzada, K. A. (2011). Reaction of exotic and indigenous capsicum genotypes against Pakistani isolates of chilli veinal mottle virus. Pakistan Journal of Botany, 43(3), 1707–1711.

  • Singh J. & Thakur M. R. (1977). Genetics of resistance to tobacco mosaic virus, cucumber mosaic virus and leaf curl virus in hot pepper (Capsicum annuum L.). Capsicum, 77.

  • Snedecor, G. W., & Cochran, W. G. (1967). Statistical methods (6th ed.). Amesterdem: The Iowa State University Press.

    Google Scholar 

  • Suzuki, K., Kuroda, T., Miura, Y., & Murai, J. (2003). Screening and field traits of virus resistant source in Capsicum spp. Plant Disease, 87, 779–783.

    Article  Google Scholar 

  • Tsai, W. S., Huang, Y. C., Zhang, D. Y., Reddy, K., Hidayat, S. H., Srithongchai, W., Green, S. K., & Jan, F. J. (2008). Molecular characterization of the CP gene and 3′UTR of Chilli veinal mottle virus from south and Southeast Asia. Plant Pathology, 57(3), 408–416.

    Article  CAS  Google Scholar 

  • Yao, M., Li, N., Wang, F., & Zhibiao, Y. E. (2013). Genetic analysis and identification of QTLs for resistance to cucumber mosaic virus in chilli pepper (Capsicum annuum L.). Euphytica, 193(2), 135–145.

    Article  CAS  Google Scholar 

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Acknowledgment

Author acknowledge Director, Indian Institute of Horticulture Research, Bangalore for providing research facilities and Department of Science and Technology, Government of India for providing fellowship for Ph.D studies of Dr. P. Naresh.

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Authors and Affiliations

  1. Central Horticultural Experiment Station, Indian Institute of Horticultural Research, Regional station, Bhubaneswar, India

    P. Naresh

  2. Division of Plant Pathology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore, Karnataka, 560089, India

    M. Krishna Reddy & P. Hema Chandra Reddy

  3. Division of Vegetable Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore, Karnataka, 560089, India

    K. Madhavi Reddy

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  1. P. Naresh
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  2. M. Krishna Reddy
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  4. K. Madhavi Reddy
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Correspondence to K. Madhavi Reddy.

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Naresh, P., Reddy, M.K., Reddy, P.H.C. et al. Screening chilli (Capsicum spp.) germplasm against Cucumber mosaic virus and Chilli veinal mottle virus and inheritance of resistance. Eur J Plant Pathol 146, 451–464 (2016). https://doi.org/10.1007/s10658-016-0930-x

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  • DOI: https://doi.org/10.1007/s10658-016-0930-x

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