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Proteome profiling of the compatible interaction between wheat and stripe rust

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Abstract

Over the last decade, comparative molecular profiling studies between compatible and incompatible plant-pathogen interactions have shown that susceptible response of the host to a pathogen requires factors that promote disease development. In this study, we examined proteome profiles during a compatible interaction between wheat and stripe rust. A 2D-LC system (ProteomeLab PF2D) was used for protein separation and to compare the proteome from infected and control samples. More than 700 protein peaks at each time point were compared between pathogen- and mock-inoculated samples. Selected proteins, with significant differences in abundance were identified by nanoLC-ESI- MS/MS and generated spectra were searched against the wheat protein databases from UniProt, and NCBI and the Puccinia database from The Broad Institute. In total, the identified proteins comprised of 62 % wheat and 38 % Pst proteins. All identified proteins were searched by bioinformatics-based algorithms to detect their subcellular localization and signal peptide motifs which have the potential to catch the candidate effector proteins. The wheat proteins were classified based on their function. Although a compatible interaction, many wheat proteins, such as antioxidants, PRs and cold-responsive proteins, are implicated in defense and stress tolerance. On the pathogen side, 64 proteins were identified, and included some important pathogenicity proteins that can play role in pathogen virulence and suppress the host defense. In addition, we discovered that nine proteins have a signal sequence and three of the hypothetical fungal proteins, PGTG_11681T0, PGTG_07231T0 and CBH50687.1, have been tentatively identified as candidate effectors.

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Acknowledgments

This study was supported by TUBITAK, COST programme 109 T293 project. We thank Kadir Akan, Ayşe Yıldız and Lütfi Çetin for their help during plant inoculation and sampling. We also thank Abdulmecit Gökçe and Yavuz Öztürk for their technical support for PF2D as well as Konca Bulut and Rahmi Büyükkeskin for their experimental assistance.

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Authors and Affiliations

  1. Faculty of Science, Department of Molecular Biology and Genetic, Yildiz Technical University, Istanbul, Turkey

    Yahya Emin Demirci & Şenay Vural Korkut

  2. Faculty of Science, Department of Molecular Biology and Genetic, Istanbul University, Istanbul, Turkey

    Cihan Inan & Nazlı Arda

  3. Faculty of Science and Arts, Department of Chemistry, Ahi Evran University, Kırşehir, Turkey

    Aslıhan Günel

  4. Faculty of Science, Department of Biology, Istanbul University, Istanbul, Turkey

    Dilara Maytalman

  5. Field Crop Research Institute, P.O Box: 226, Lodumlu, Ankara, Turkey

    Zafer Mert

  6. Department of Medical Biochemistry, School of Medicine, Acibadem University, Istanbul, Turkey

    A. Tarık Baykal

  7. Faculty of Engineering, Department of Genetics and Bioengineering, Trakya University, Edirne, Turkey

    Semra Hasançebi

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  1. Yahya Emin Demirci
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Third dimension seperation of proteins by 12 % 1D-PAGE (JPEG 131 kb)

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Demirci, Y.E., Inan, C., Günel, A. et al. Proteome profiling of the compatible interaction between wheat and stripe rust. Eur J Plant Pathol 145, 941–962 (2016). https://doi.org/10.1007/s10658-016-0882-1

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