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The widespread occurrences of Beet soil borne virus and RNA-5 containing Beet necrotic yellow vein virus isolates in sugar beet production areas in Turkey

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Abstract

A total of 632 soil samples were collected from different sugar beet fields in 32 provinces during 2005, 2010 and 2011 growing seasons. Incidences of Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus (BSBV) and their vector Polymyxa betae were determined by bait plant tests using ELISA and root staining techniques. The percentages of BNYVV and BSBV infested soil samples were 38 and 49.8 %, respectively. The study showed that P. betae was very common (87.7 %) in the sampled sugar beet fields. Additionally, the presence of BNYVV RNA-5 component was investigated by RT-PCR using the primers specific for p26 coding region. Out of 240 BNYVV-infected samples, 85 were selected according to their geographic origin, and 62.4 % of them belonging to 29 provinces were found to contain the RNA-5 segment. The partial nucleotide sequences of RNA-5 of BNYVV isolates from Igdir, Edirne, Ankara and Konya provinces of Turkey were obtained. Nucleotide sequencing and phylogenetic analyses showed that RNA-5 of Turkish BNYVV isolates is closer to the J type BNYVV isolates than the P type isolates. These results also indicated that BNYVV populations containing RNA-5 is highly widespread in sugar beet production areas in Turkey.

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Acknowledgments

We are grateful to Dr. Claude Bragard for kindly providing soil samples infected with BNYVV P type and Dr. Islam Gunduz for great help with phylogenetic analysis.

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Plant Protection, Ondokuz Mayis University, Samsun, 55139, Turkey

    Nazli D. Kutluk Yilmaz, Miray Arli Sokmen, Mehmet Ali Sevik & Berna Tunali

  2. Department of Phytopathology, Sugar Institute, Ankara, TR-06930, Turkey

    Riza Kaya

  3. Faculty of Arts and Science, Department of Biology, Ondokuz Mayis University, 55139, Samsun, Turkey

    Sadık Demirtaş

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  1. Nazli D. Kutluk Yilmaz
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Correspondence to Nazli D. Kutluk Yilmaz.

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The work presented has been funded by The Scientific and Technological Research Council of Turkey (TUBITAK), The Grant Number: TOVAG 110O188. This article does not contain any studies with human participants or animals.

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Yilmaz, N.D.K., Sokmen, M.A., Kaya, R. et al. The widespread occurrences of Beet soil borne virus and RNA-5 containing Beet necrotic yellow vein virus isolates in sugar beet production areas in Turkey. Eur J Plant Pathol 144, 443–455 (2016). https://doi.org/10.1007/s10658-015-0780-y

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