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Impact of abiotic stresses on the protection efficacy of defence elicitors and on metabolic regulation in tomato leaves infected by Botrytis cinerea

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Abstract

Finding sustainable plant protection strategies is a major challenge for agriculture. Taking advantage of the plant natural immune system by using plant defence elicitors is an interesting avenue to explore. However, transfer to field application is often difficult, mostly due to the complexity of interactions between plants and their environment, involving biotic and abiotic stresses. The protection efficacy against gray mold and the modes of action of potential elicitors were studied on tomato. Modulation of plant defense was studied using both global and targeted metabolic profiling. We identified seven potential elicitors showing good plant protection efficacy and able to trigger the oxylipin pathway, including jasmonic acid production, after inoculation with Botrytis cinerea. Following preliminary assays, seven elicitors including two well-studied elicitors (Bion 50WG® and BABA) showing good plant protection efficacy and low fungitoxic effect were selected to assay the effect of abiotic stresses (wounding, water stress and nitrogen deficiency) on their protection efficacy. Our results showed that the protection efficacy of all products was reduced when plants were exposed to abiotic stresses, suggesting an antagonistic interaction between the tomato responses to abiotic stresses and product treatments. We found that responses to leaf cuttings and product treatments induced metabolic changes in a time-dependent manner, and that both of which mainly activated the oxylipin and JA pathway. However, the negative effects of wounding on tomato protection efficacy of defence elicitors suggest that interplay with other antagonistic signalling pathways is also involved in the tomato responses to this combination of stress.

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Acknowledgment

This work was financially supported by Vegenov-BBV, GCSAR (General Commission for Scientific Agricultural Research-Syria) and the French Ministry of Agriculture. It was also supported by companies providing the different PDE products. We are grateful to the plant pathology team in Vegenov for their technical assistance and their advices.

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Author notes

  1. Picot Adeline

    Present address: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280, Plouzané, France

  2. Charton Sophie

    Present address: Centre de Recherche Public Gabriel Lippmann, Département EVA, Plateforme de Protéomique et de Métabolomique Végétale, 4422, Belvaux, Luxemburg

  3. Hallier Sonia

    Present address: , Kermorvan, 29410, Guiclan, France

Authors and Affiliations

  1. Vegenov-BBV, Penn ar Prat, 29250, St-Pol-de-Léon, France

    Aljabal Maymoune, Picot Adeline, Turner Marie & Hallier Sonia

  2. Sorbonne Universités, UPMC University of Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, 29688, Roscoff CEDEX, France

    Goulitquer Sophie, Charton Sophie & Leblanc Catherine

  3. CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, 29688, Roscoff CEDEX, France

    Goulitquer Sophie, Charton Sophie & Leblanc Catherine

  4. Montpellier Supagro, UMR BGPI - TA A-54/K, Campus International de Baillarguet, 34398, Montpellier Cedex 5, France

    Neema Claire

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  1. Aljabal Maymoune
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Correspondence to Turner Marie.

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Maymoune, A., Adeline, P., Marie, T. et al. Impact of abiotic stresses on the protection efficacy of defence elicitors and on metabolic regulation in tomato leaves infected by Botrytis cinerea . Eur J Plant Pathol 142, 223–237 (2015). https://doi.org/10.1007/s10658-015-0606-y

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