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A begomovirus associated with yellow vein symptoms of Oxalis debilis

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Abstract

‘Gold Veined Oxalis’ (Oxalis debilis) is an ornamental plant cultivar with attractive yellow vein foliage. After grafting scions of this plant to O. debilis plants that lacked the yellow vein foliage, grafted plants developed yellow vein suggesting that an infectious agent was the cause of the yellow vein. DNA extracts from O. debilis plants showing yellow vein were used in rolling circle amplification and PCR experiments. Sequence analyses of amplified DNA products suggested that a begomovirus was the cause of the yellow vein symptoms. The same begomovirus was found in the original sample and in graft inoculated plants that developed the yellow vein symptoms. The DNA A of the putative begomovirus, designated Oxalis yellow vein virus (OxYVV), shared 87 % overall nucleotide identities with the corresponding genome segments of Sida mottle virus, 85 % with Okra mottle virus, and 83 % with Tomato yellow spot virus. OxYVV virus was transmitted by the whitefly Bemisia tabaci Middle East-Asia Minor 1. This is another example of a virus that enhances the aesthetics of an ornamental plant.

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Fig. 1
Fig. 2

Abbreviations

AbMV:

Abutilon mosaic virus

ClGMCNV:

Clerodendron golden mosaic China virus

HYVMV:

Honeysuckle yellow vein mosaic virus

HYVV:

Honeysuckle yellow vein virus

OxYVV:

Oxalis yellow vein virus

MEAM1:

Middle East-Asia Minor 1

OMoV:

Okra mottle virus

PCR:

Polymerase chain reaction

RCA:

Rolling circle amplification

SimMV:

Sida micrantha mosaic virus

SiMBV:

Sida mosaic Brazil virus

SiMoV:

Sida mottle virus

TYLCV-IL:

Tomato yellow leaf curl virus-Israel

ToYSV:

Tomato yellow spot virus

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Acknowledgments

The authors wish to thank Sead Sabanadzovic (Mississippi State University) and Andrea Hebert (Louisiana State University) for providing valuable suggestions to the manuscript. This research was partially supported by funds from the LSU AgCenter Zamorano Scholars Program, the Mississippi Agriculture and Forestry Experiment Station and the USDA National Institute of Food and Agriculture.

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Authors and Affiliations

  1. Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA, 70803, USA

    Favio Herrera & Rodrigo A. Valverde

  2. Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS, 39762, USA

    Nina Aboughanem-Sabanadzovic

Authors
  1. Favio Herrera
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  2. Nina Aboughanem-Sabanadzovic
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  3. Rodrigo A. Valverde
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Corresponding author

Correspondence to Rodrigo A. Valverde.

Additional information

Sequence reported in this paper has been deposited in the GenBank under accession number KM887907

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Herrera, F., Aboughanem-Sabanadzovic, N. & Valverde, R.A. A begomovirus associated with yellow vein symptoms of Oxalis debilis . Eur J Plant Pathol 142, 203–208 (2015). https://doi.org/10.1007/s10658-015-0594-y

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