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Olive mild mosaic virus transmission by Olpidium virulentus

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Abstract

The ability of Olpidium virulentus to vector Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNV-D) was evaluated. Transmission assays involved zoospore acquisition of each virus, inoculation onto cabbage plant roots followed by viral detection. Assays revealed that these viruses are transmitted in the absence of the fungus, but the transmission rates of OMMV are much higher when OMMV is incubated with O. virulentus zoospores prior to inoculation, while the transmission rates of each OLV-1 and TNV-D do not change when they are incubated with the fungus. Our data shows that O. virulentus is an efficient vector of OMMV, greatly enhancing virus transmissibility, but not that of OLV-1 nor TNV-D. Prior studies have shown that another fungus, O. brassicae, also enhances OMMV transmission. To our knowledge, this is the first report of a plant virus, OMMV, being efficiently transmitted by two different species of fungi.

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Acknowledgments

The authors would like to thank José Ángel Herrera-Vásquez (Universidad Politécnica de Valencia, Spain) for providing DNA from O. brassicae and O. virulentus to use as controls. Carla MR Varanda is recipient of a PhD fellowship from FCT, SFRH/BPD/76194/2011, financed by QREN - POPH - Typology 4.1 - co-financed by MES national funding and The European Social Fund. This work has been supported by FEDER and National funds, through the Programa Operacional Regional do Alentejo (InAlentejo) Operation ALENT-07-0262-FEDER-001871/ Laboratório de Biotecnologia Aplicada e Tecnologias Agro-Ambientais.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Laboratório de Virologia Vegetal, Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, 7002-554, Évora, Portugal

    Carla M. R. Varanda, Susana Santos, Maria Ivone E. Clara & Maria do Rosário Félix

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  1. Carla M. R. Varanda
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  2. Susana Santos
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  3. Maria Ivone E. Clara
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  4. Maria do Rosário Félix
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Correspondence to Carla M. R. Varanda.

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Varanda, C.M.R., Santos, S., Clara, M.I.E. et al. Olive mild mosaic virus transmission by Olpidium virulentus . Eur J Plant Pathol 142, 197–201 (2015). https://doi.org/10.1007/s10658-015-0593-z

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