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Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China

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Abstract

This is the first report of the complete genome sequence of Pineapple mealybug wilt-associated virus-1 (PMWaV-1 HN, KJ872494) identified on pineapple [Ananas comosus (L.) Merr.] in Hainan island, China. The genome is 13,069 nucleotides long, possessing seven open reading frames (ORFs). The ORF1 encodes a 233 kDa polyprotein which has PRO protease domain, MTR methyltransferase domain and HEL helicase domain; The ORF2 ~ ORF7 encode RNA-dependent RNA polymerase protein (RdRp), a 6 kDa protein (p6), heat shock 70 homolog protein (HSP70h), a 46 kDa protein (p46), coat protein (CP) and a 24 kDa protein (p24), respectively. Full-length nucleotide sequence analysis of PMWaV-1 HN genome confirmed it 97.99 % homology with PMWaV-1 (AF414119) which was isolated from Hawaii, USA. Furthermore, based on Maximum Likelihood method, all conserved trees of RdRp, HSP70h and CP proteins sequences revealed that PMWaV-1 HN together with other PMWaV-1 isolates clearly separated into PMWaV-1 group.

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Fig. 1

Abbreviations

PMWaV-1:

Pineapple mealybug wilt-associated virus-1

PMWaV-2:

Pineapple mealybug wilt-associated virus-2

PMWaV-3:

Pineapple mealybug wilt-associated virus-3

MWP:

Mealybug wilt of pineapple

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Acknowledgments

This work was supported by the Natural Science Fund of Hainan Province (NO.313096), the Scientific Research Fund of Hainan Province (KYYS-2013-02) and the Major Science and Technology Program of Hainan Province (ZDZX2013023-1).

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Authors and Affiliations

  1. Institute of Tropical Fruit Tree, Hainan Academy of Agricultural Science/Haikou Investigation Station of Tropical Fruit Trees, Ministry of Agriculture, Hainan, Haikou, 571100, China

    Naitong Yu, Zhiwen Luo, Hongyan Fan, Zhili Zhang, Xianghong Li & Fan He

  2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China

    Naitong Yu, Jianhua Wang & Zhixin Liu

Authors
  1. Naitong Yu
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  2. Zhiwen Luo
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  3. Hongyan Fan
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  4. Zhili Zhang
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  5. Xianghong Li
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  6. Jianhua Wang
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  7. Zhixin Liu
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  8. Fan He
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Corresponding authors

Correspondence to Zhixin Liu or Fan He.

Additional information

Naitong Yu and Zhiwen Luo contributed equally to this work.

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Yu, N., Luo, Z., Fan, H. et al. Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China. Eur J Plant Pathol 141, 611–615 (2015). https://doi.org/10.1007/s10658-014-0545-z

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