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Fusarium oxysporum f. sp. lycopersici retardation through induction of defensive response in tomato plants using a liquid formulation of Pseudomonas fluorescens (Pf1)

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Abstract

A liquid based Pseudomonas fluorescens (Pf1) bioformulation was found to contribute the restriction of Fusarium oxysporum f. sp. lycopersici in tomato roots by inducing defence enzymes. Induction of defence enzymes such as phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenoloxidase (PPO), catalase, β-1,3 glucanase and super oxide dismutase (SOD), was studied in tomato plants pretreated with liquid as well as a talc based formulation of Pf1 challenged with F. oxysporum f. sp. lycopersici in glasshouse vegetable production systems. There were increased activities of PAL, PO, PPO, catalase and β-1 3-glucanases in tomato plants treated with a combined application of seedling dip + soil application + foliar spray of liquid and talc formulation of Pf1 when compared to pathogen inoculated and untreated healthy controls. The activities of the above enzymes started to increase at 3rd day, reached maximum levels on 8-9th day and thereafter declined gradually. Similarly, native polyacralamide gel electrophoresis (PAGE) analysis revealed that one to six isoforms of the defence enzymes each with a higher intensity were expressed in these treatments, whereas fewer isoforms with less intensity were noticed in inoculated controls. These results suggest that the retardation of the invasion of F. oxysporum f. sp. lycopersici in tomato roots resulting from treatment with the liquid formulation of Pf1 was due to enhancement of activities of enzymes involved in the phenylpropanoid pathway. These results suggest that induced systemic resistance occurred in the treated tomato plants.

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  1. Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641003, India

    R. Manikandan & T. Raguchander

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Manikandan, R., Raguchander, T. Fusarium oxysporum f. sp. lycopersici retardation through induction of defensive response in tomato plants using a liquid formulation of Pseudomonas fluorescens (Pf1). Eur J Plant Pathol 140, 469–480 (2014). https://doi.org/10.1007/s10658-014-0481-y

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