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New evidence of intra-race diversity in Fusarium oxysporum f. sp. dianthi populations based on Vegetative Compatibility Groups

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Abstract

Fusarium oxysporum f. sp. dianthi (Fod) causes vascular wilt, the most important carnation disease worldwide. We have analyzed vegetative compatibility in a collection of Fod isolates, obtained from both soils and carnation plants in the most important growing areas in Spain, by pairing all isolates in all possible combinations. Results showed that isolates of race 1 and race 2 were distributed among three Vegetative Compatibility Groups (VCG) which correlated with the molecular Groups previously described. Isolates of race 1 and race 2 in molecular Group I grouped in VCG 0021, isolates of race 1 type were in VCG 0022, and isolates of race 1 and race 2 in molecular Group II constituted a new VCG (002-), not previously reported. Isolates in each VCG contained the same mating type gene (MAT1-1 or MAT1-2), with the exception of the new VCG 002- that contained both idiomorphs. This work identifies a new VCG in Fod populations and reports for the first time the presence of isolates of race 1 and race 2 in the same VCG.

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Acknowledgments

This research was supported by Grants AGL 2006–03252 and AGL 2010–18279 from the Spanish Ministry of Science and Innovation. We thank Carlos Casanova and Antonio Valverde for their valuable technical assistance. We also thank Dr. M. Dolores García-Pedrajas for reviewing the manuscript.

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  1. Department of Crop Protection, Instituto de Agricultura Sostenible, IAS-CSIC, Alameda del Obispo s/n. Apdo 4084, 14080, Córdoba, Spain

    Carmen Gómez-Lama Cabanás & Encarnación Pérez-Artés

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  1. Carmen Gómez-Lama Cabanás
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  2. Encarnación Pérez-Artés
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Correspondence to Encarnación Pérez-Artés.

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Gómez-Lama Cabanás, C., Pérez-Artés, E. New evidence of intra-race diversity in Fusarium oxysporum f. sp. dianthi populations based on Vegetative Compatibility Groups. Eur J Plant Pathol 139, 445–451 (2014). https://doi.org/10.1007/s10658-014-0412-y

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