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Host status of barley to Puccinia coronata from couch grass and P. striiformis from wheat and brome

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Abstract

The pathogenicity and identity was studied of a field sample (PcE) of crown rust fungus Puccinia coronata collected in Hungary on wild couch grass (Elymus repens) and of a field sample (Psb) of stripe rust (P. striiformis) collected in the Netherlands on California brome (Bromus carinatus). We focused on the analysis of the host susceptibility of cultivated barley (Hordeum vulgare) to both pathogen samples, in direct comparison with an isolate of P. striiformis f.sp. tritici (Pst) and P. striiformis f.sp. hordei (Psh). At the seedling stage 83 % of the barley accessions were susceptible to PcE. At the adult plant stage, cultivated barley was predominantly resistant to this crown rust sample. Morphology, pathogenicity on Hordeum and Bromus and ITS DNA sequence analysis determined that PcE represents a European specimen of P. coronata f.sp. hordei. Sample Psb was pathogenic on 47 % of the cultivated barley at the seedling stage. This is intermediate between the isolates Psh (90 %) and Pst (10 %). Psb and Psh were pathogenic to grasses representing several genera. The host range of the Pst isolate was more narrow. We conclude that Psb is unlikely to represent a genotype of Psh that happened to have colonized B. carinatus, and is likely a distinct form of P. striiformis.

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Acknowledgments

We thank Dr. Tatjana Oja of the Institute of Botany and Ecology at the University of Tartu, Estonia, for kindly providing the seeds of the many Bromus accessions, and also express our gratitude to Adriaan van Aelst, WUR, Wageningen, Netherlands, for his kind help in making the SEM photographs of the telia in Fig. 1 and K. P. Nguyen (USDA ARS CDL) for technical assistance in DNA analysis. Mention of a trademark name or proprietary product does not constitute a guarantee by the U.S. Department of Agriculture.

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Author notes

  1. Skye van Heyzen

    Present address: KWS-UK Ltd., Thriplow, Royston, SG8 7RE, Hertfordshire, UK

  2. Sisay K. Alemu

    Present address: Agricultural Biotechnology Research Program, Ethiopian Institute of Agricultural Research, Holetta Research Center, Holetta, Ethiopia

Authors and Affiliations

  1. Laboratory of Plant Breeding, Graduate school for Experimental Plant Sciences, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Rients E. Niks, Skye van Heyzen & Sisay K. Alemu

  2. USDA-ARS Cereal Disease Laboratory, University of Minnesota, 1551 Lindig Street, St. Paul, MN, USA

    Les J. Szabo

Authors
  1. Rients E. Niks
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  2. Skye van Heyzen
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  3. Les J. Szabo
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  4. Sisay K. Alemu
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Corresponding author

Correspondence to Rients E. Niks.

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Supplementary Figure 1

Phylogenetic analysis of PcE and Psb based on ITS and 5′ LSU rDNA sequence data. A. Analysis of PcE with representative sequences of the Puccinia coronata complex; B. Analysis of Psb with representative sequences of the P. striiformis complex. Numbers above or below branches indicate percentage of congruent clusters in 5000 bootstrap trials, and only values above 75% are shown. DNA sequence of P. graminis (AY114289) was used as an outgroup. (PDF 103 kb)

Supplementary Table 1

List of barley accessions (at seedling stage) used for the host status quantification of Puccinia coronata collected from Elymus repens. (DOCX 185 kb)

Supplementary Table 2

List of barley accessions (at seedling stage) used for the host status quantification to Puccinia striiformis collected from Bromus carinatus (Psb), wheat (Pst) and barley (Psh). (DOCX 2031 kb)

Supplementary Table 3

List of species and accessions tested for host range determination of Puccinia coronata collected from Elymus repens. (DOCX 116 kb)

Supplementary Table 4

List of species and accessions tested for host range determination of Puccinia striiformis collected from Bromus carinatus (Psb), wheat (Pst) and barley (Psh). (DOCX 1531 kb)

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Niks, R.E., van Heyzen, S., Szabo, L.J. et al. Host status of barley to Puccinia coronata from couch grass and P. striiformis from wheat and brome. Eur J Plant Pathol 136, 393–405 (2013). https://doi.org/10.1007/s10658-013-0174-y

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