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Effect of plant elicitors on the reproduction of the root-knot nematode Meloidogyne chitwoodi on susceptible hosts

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Abstract

Sustainable strategies for plant-parasitic nematode control are required to reduce dependence on chemical nematicides. Foliar application of various compounds can induce a systemic defence response that reduces nematode infestation. The effects of benzothiadiazole (BTH), β-aminobutyric acid (BABA), jasmonates (cis-jasmone and methyl jasmonate) and salicylic acid (SA) in the development and reproduction of the root-knot nematode Meloidogyne chitwoodi in tomato plants were assessed. The effects of BTH and of the jasmonates were further tested on potato plants. Pot assays were conducted using tomato plants cv. Tiny Tim or potato cv. Désirée treated with foliar sprays and inoculated with 300 second stage juveniles. Nematode development and reproduction were assessed 21 and 45 days after inoculation. Treatment with SA had a negative effect on nematode development in tomato plants but did not affect reproduction and methyl jasmonate treatment was the most effective in reducing nematode penetration (58 %). Nematode development was significantly affected in potato plants sprayed with cis-jasmone. Nematode penetration was reduced by 90, 67 and 81 % in plants treated with BTH, cis-jasmone and methyl jasmonate respectively, although the reproduction factor (Rf) was only significantly lower in the BTH treatment (Rf = 7.6) when compared to the control (Rf = 18.1). Our results suggest that both the SA and JA pathways play an important role in plant defence mechanisms against root-knot nematode development and reproduction for both plants, and should be considered in the design of integrated pest management approaches.

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Acknowledgments

The first author was supported by ESF funds through the “Programa Operacional Potencial Humano - POPH” of the “The National Strategic Reference Framework” (SFRH/BD/27317/2006). Rothamsted Research receives grant aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom.

We thank Barry Pye for spraying the tomato plants using the Rothamsted Research Experimental Station automatic hydraulic sprayer.

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Authors and Affiliations

  1. IMAR-CMA, Department of Life Sciences, University of Coimbra, 3004-517, Coimbra, Portugal

    M. C. Vieira dos Santos & I. Abrantes

  2. AgroEcology Department, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    M. C. Vieira dos Santos & R. H. C. Curtis

Authors
  1. M. C. Vieira dos Santos
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  2. R. H. C. Curtis
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  3. I. Abrantes
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Correspondence to M. C. Vieira dos Santos.

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Vieira dos Santos, M.C., Curtis, R.H.C. & Abrantes, I. Effect of plant elicitors on the reproduction of the root-knot nematode Meloidogyne chitwoodi on susceptible hosts. Eur J Plant Pathol 136, 193–202 (2013). https://doi.org/10.1007/s10658-012-0155-6

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  • DOI: https://doi.org/10.1007/s10658-012-0155-6

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