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Characterisation and pathogenicity of Pestalotiopsis uvicola and Pestalotiopsis clavispora causing grey leaf spot of mango (Mangifera indica L.) in Italy

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Abstract

During 2009 and 2010, twenty-one isolates of Pestalotiopsis spp. Associated with grey patches on the leaves, twigs, and panicles of mango were collected in six orchards located in Sicily (Italy). Morphological characteristics of colony (colour and mycelium appearance), and conidia (size, shape, septation, length and the number of apical and the basal appendages) as well as phylogenetic analysis of the Internal Transcribed Spacer (ITS) region (ITS1, 5.8S gene, and ITS2) of six representative isolates revealed the occurrence of P. uvicola and P. clavispora. The representative isolates of both species were pathogenic to the artificially inoculated detached mango leaves cv. Kensington Pride and showed significant variation in lesion size. This is the first report worldwide of P. uvicola and P. clavispora causing grey leaf spot of mango.

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Acknowledgments

The authors thank T. Yasseen for helpful contribution to collection of plant samples and G. Scuderi and R. Faedda for techical assistance in molecular analysis of the strains.

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Correspondence to Giancarlo Polizzi.

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Ismail, A.M., Cirvilleri, G. & Polizzi, G. Characterisation and pathogenicity of Pestalotiopsis uvicola and Pestalotiopsis clavispora causing grey leaf spot of mango (Mangifera indica L.) in Italy. Eur J Plant Pathol 135, 619–625 (2013). https://doi.org/10.1007/s10658-012-0117-z

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