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Physiological response of Bacillus cereus C1L-induced systemic resistance in lily against Botrytis leaf blight

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Abstract

Bacillus cereus C1L has been demonstrated to induce systemic disease resistance against Botrytis elliptica in lily. The objective of this study was to investigate physiological responses of B. cereus C1L-triggered systemic resistance in lily cv. Star Gazer against B. elliptica. By histological and biochemical analyses, leaves inoculated with B. elliptica displayed cell death, H2O2 accumulation and lignin deposition. As plants were elicited with B. cereus C1L, cell death, H2O2 accumulation and lignin deposition in leaves caused by B. elliptica infection were suppressed, revealing that suppression of oxidative burst might be associated with B. cereus C1L-induced systemic resistance. In reactive oxygen species inhibitors assays, B. elliptica-caused lesion numbers and H2O2 accumulation in lily leaves were significantly reduced as leaves were pretreated with catalase or diphenylene iodonium. Furthermore, the expression of LsGRP1 and LsPsbR in leaves elicited with B. cereus C1L and inoculated with B. elliptica was decreased. The same expression pattern was also observed in leaves pretreated with catalase or diphenylene iodonium and inoculated with B. elliptica. These results suggest that B. cereus C1L-induced systemic resistance may be related to suppression or alleviation of oxidative stress and cell death of lily caused by B. elliptica.

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Abbreviations

ROS:

reactive oxygen species

ISR:

induced systemic resistance

CAT:

catalase

DPI:

diphenylene iodonium

dai:

days after inoculation

hpi:

hours post inoculation

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Acknowledgements

This study was supported by the National Science Council grants 96-2317-B-002-001 and 97-2317-B-002-001 to C. Y. Chen and by the Aim for Top University Project of National Taiwan University grants 99R40044 and 10R40044 to C. J. Huang.

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Authors and Affiliations

  1. Department of Plant Pathology and Microbiology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan, Republic of China

    Chien-Jui Huang, Yi-Hung Liu, Ken-Haow Yang & Chao-Ying Chen

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  1. Chien-Jui Huang
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  2. Yi-Hung Liu
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  3. Ken-Haow Yang
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  4. Chao-Ying Chen
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Correspondence to Chao-Ying Chen.

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Chien-Jui Huang and Yi-Hung Liu contributed equally.

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Huang, CJ., Liu, YH., Yang, KH. et al. Physiological response of Bacillus cereus C1L-induced systemic resistance in lily against Botrytis leaf blight. Eur J Plant Pathol 134, 1–12 (2012). https://doi.org/10.1007/s10658-012-0013-6

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