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Discrimination of Xanthomonas campestris pv. campestris races among strains from northwestern Spain by Brassica spp. genotypes and rep-PCR

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Abstract

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is a severe seedborne disease of Brassica crops around the world. Nine races are recognized, being races 1 and 4 the most aggressive and widespread. The identification of Xcc races affecting Brassica crops in a target area is necessary to establish adequate control measures and breeding strategies. The objectives of this study were to isolate and identify Xcc strains from northwestern Spain by using semi-selective medium and pathogenicity tests, determine the existing races of Xcc in this area by differential series of Brassica spp., and evaluate the use of repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) to differentiate among the nine existing Xcc races. Seventy five isolates recovered from infected fields were identified as Xcc. Race-typing tests determined the presence of the following seven pathogen races: 1, 4, 5, 6, 7, 8 and 9. Race 4 was the most frequent in Brassica oleracea and race 6 in Brassica rapa crops, therefore breeding should be focussed in obtaining resistant varieties to both races. Cluster analysis derived from the combined fingerprints showed four groups, but no clear relationship to race, crop or geographical origin was found. Rep-PCR analysis was found not to be a reliable method to discriminate among Xcc races, therefore race typing of Xcc isolates should be done by using the differential series of Brassica spp. genotypes or another alternative approach.

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Acknowledgements

The authors thank to M.M. López (IVIA, Valencia, Spain) for supplying five bacterial strains from Spain, to Joana Vicente from Warwick HRI, Wellesbourne (UK) for supplying the Brassica differential series for Xanthomonas campestris pv. campestris and the bacterial strains HRI 3811, HRI 3849A, HRI 5212, HRI 1279A, HRI 3880 and HRI 6181 representing races 1 to 6, and to CFBF-INRA, Angers (France) for supplying bacterial strains CFBP 4953, CFBP 1124 and CFBP 6650 representing races 7, 8 and 9 respectively. Authors also thank to Eva González her help in DNA extractions.

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  1. Department of Plant Genetics, Misión Biológica de Galicia (MBG-CSIC), P.O. Box 28, 36080, Pontevedra, Spain

    Margarita Lema, María Elena Cartea, Tamara Sotelo, Pablo Velasco & Pilar Soengas

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  1. Margarita Lema
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  2. María Elena Cartea
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  3. Tamara Sotelo
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  4. Pablo Velasco
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  5. Pilar Soengas
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Correspondence to Pilar Soengas.

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Lema, M., Cartea, M.E., Sotelo, T. et al. Discrimination of Xanthomonas campestris pv. campestris races among strains from northwestern Spain by Brassica spp. genotypes and rep-PCR. Eur J Plant Pathol 133, 159–169 (2012). https://doi.org/10.1007/s10658-011-9929-5

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