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Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine

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Abstract

A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ -resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR.

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Acknowledgments

This project was funded by the National Centre of Competence in Research (NCCR) Plant Survival, a research programme of the Swiss Science Foundation, and supported by European Cooperation in the field of Scientific and Technical Research (COST) Action 858 and by a grant from the State Secretariat for Education and Research (Switzerland).

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Correspondence to Eliane Abou-Mansour.

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Djoukeng, J.D., Polli, S., Larignon, P. et al. Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine. Eur J Plant Pathol 124, 303–308 (2009). https://doi.org/10.1007/s10658-008-9419-6

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  • DOI: https://doi.org/10.1007/s10658-008-9419-6

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