Abstract
A new disease was observed on Trifolium dasyurum, with symptoms beginning as a halo spot and developing into a leaf blight. The causal organism was identified by microscopy and DNA sequence studies as Botrytis fabae. This strain of B. fabae was also demonstrated to cause disease on foliage of a range of pulse crops, including Vicia faba, Pisum sativum, and Lens culinaris. This study demonstrates the potential of this strain of B. fabae to not only pose a significant threat to T. dasyurum but also to pulses grown in rotation with T. dasyurum that are susceptible to this strain of B. fabae.
References
Barbetti, M. J. (1987). Effects of three foliar diseases on biomass and seed yield for 11 cultivars of subterranean clover. Plant Disease, 71, 350–353.
Barbetti, M. J., & Nichols, P. G. H. (2005). Field performance of subterranean clover germplasm in relation to severity of Cercospora disease. Australasian Plant Pathology, 34, 197–201.
Coley-Smith, J. R., Verhoeff, K., & Jarvis, W. R. (1980). The biology of Botrytis. London, UK: Academic.
Davidson, J. A., Pande, S., Bretag, T. W., Lindbeck, K. D., & Krishna-Kishore, G. (2004). Biology and management of Botrytis spp. in legume crops. In Y. Elad, B. Williamson, P. Tudzynski, & N. Delen (Eds.), Botrytis: biology, pathology and control (pp. 295–318). Dordrecht, The Netherlands: Kluwer.
Deverall, B. J., & Wood, R. K. S. (1968). Disease resistance in Vicia faba and Phaseolus vulgaris. Netherlands Journal of Plant Pathology, 74(supplement 1), 137–148.
Ellis, M. B., & Waller, J. M. (1974). Botrytis fabae. In: IMI descriptions of fungi and bacteria sheet 432. Issued by the Commonwealth Mycological Institute, Ferry Lane, Kew, Surrey England, Eastern Press Ltd., London and Reading
Graham, P. H., & Vance, C. P. (2003). Legumes: Importance and constraints to greater use. Plant Physiology, 131, 872–877.
Hanounik, S. B., & Maliha, N. (1986). Horizontal and vertical resistance in Vicia faba to chocolate spot caused by Botrytis fabae. Plant Disease, 70, 770–773.
Holz, G., Coertze, S., & Williamson, B. (2004). The ecology of botrytis on plant surfaces. In Y. Elad, B. Williamson, P. Tudzynski, & N. Delen (Eds.), Botrytis: biology, pathology and control (pp. 9–27). Dordrecht, The Netherlands: Kluwer.
Hutson, R. A., & Mansfield, J. W. (1980). A genetical approach to the analysis of mechanisms of pathogenicity in Botrytis/Vicia faba interactions. Physiological Plant Pathology, 17, 309–317.
Jarvis, W. R. (1977). Botryotinia and Botrytis species: Taxonomy, physiology, and pathogenicity, A guide to the literature, Monograph No. 15. Ottawa, Canada: Canada Department of Agriculture.
Loi, A., Nutt, B. J., Revell, C. K., & Snowball, R. (2007). AGWEST Sothis: Trifolium dasyurum (eastern star clover). Australian Journal of Experimental Agriculture, 47, 1512–1515.
Mansfield, J. W., & Hutson, R. A. (1980). Microscopical studies on fungal development and host responses in broad bean and tulip leaves inoculated with five species of Botrytis. Physiological Plant Pathology, 1, 131–144.
Nichols, P. G. H., Loi, A., Nutt, B. J., Evans, P. M., Craig, A. D., Pengellym, B. C., et al. (2007). New annual and short-lived perennial pasture legumes for Australian agriculture–15 years of revolution. Field Crops Research, 104, 10–23.
Raeder, U., & Broda, P. (1985). Rapid preparation of DNA from filamentous fungi. Letters of Applied Microbiology, 1, 17–20.
Staats, M., Baarlen, P., & van Kan, J. A. L. (2005). Molecular phylogeny of the plant pathogenic genus Botrytis and evolution of host specificity. Molecular Biology and Evolution, 22, 333–346.
Tivoli, B., Baranger, A., Avila, C. M., Banniza, S., Barbetti, M. J., Chen, W., et al. (2006). Screening techniques and sources of resistance to foliar diseases caused by major necrotrophic fungi in grain legumes. Euphytica, 147, 223–253.
USDA, ARS, National Genetic Resources Program (2008). Germplasm Resources Information Network—(GRIN) [Online Database]. National Germplasm Resources Laboratory, Beltsville, Maryland. Checked from URL: http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?40209.
Verhoeff, K., Malathrakis, N. E., & Williamson, B. (1992). Recent advances in Botrytis research. Wageningen The Netherlands: Pudoc Scientific Publishers.
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We thank the Department of Agriculture and Food Western Australia and the Schools of Plant Biology and Earth and Geographical Sciences at The University of Western Australia for funding this research.
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You, M.P., Yang, H.A., Sivasithamparam, K. et al. A new leaf blight disease of Trifolium dasyurum caused by Botrytis fabae . Eur J Plant Pathol 123, 99–103 (2009). https://doi.org/10.1007/s10658-008-9338-6
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DOI: https://doi.org/10.1007/s10658-008-9338-6