European Journal of Plant Pathology

, Volume 120, Issue 1, pp 69–77

Chitinase levels in rice cultivars correlate with resistance to the sheath blight pathogen Rhizoctonia solani

  • C. L. Shrestha
  • I. Oña
  • S. Muthukrishnan
  • T. W. Mew
Full Research Paper

DOI: 10.1007/s10658-007-9199-4

Cite this article as:
Shrestha, C.L., Oña, I., Muthukrishnan, S. et al. Eur J Plant Pathol (2008) 120: 69. doi:10.1007/s10658-007-9199-4

Abstract

Various rice cultivars were selected and screened for their reaction to sheath blight in the greenhouse. Cluster analysis of percent relative lesion height (% RLH) generated four groups of cultivars with a coefficient of similarity of 3.27. Chitinase activities were detected 24 h after inoculation of moderately resistant cvs Betichikon, Dudruchi, Khatochalani, Padi Pulut Malat, Kakua, IR72, Khakibinni. But in the susceptible cv. IR58, chitinase activity was detected only 36 h after inoculation. Western blot analysis showed that class 1 and class 2 chitinases were induced following Rhizoctoniasolani infection of these cultivars. The % RLH and the number of infection cushions were negatively correlated with the level of chitinase activity. Moderately resistant rice cultivars had higher levels of chitinase activity and lower disease severity and numbers of infection cushions formed compared to IR58.

Keywords

ChitinasesDisease resistanceRiceSheath blight

Copyright information

© KNPV 2007

Authors and Affiliations

  • C. L. Shrestha
    • 1
  • I. Oña
    • 1
  • S. Muthukrishnan
    • 2
  • T. W. Mew
    • 1
  1. 1.Entomology and Plant Pathology DivisionInternational Rice Research Institute (IRRI)Los BañosPhilippines
  2. 2.Department of BiochemistryKansas State UniversityManhattanUSA