Date: 19 Sep 2007
Horizontal transmission of hypoviruses between vegetative compatibility types of Cryphonectria parasitica in Macedonia
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Biological control of chestnut blight with hypovirulence depends on the successful transmission of hypoviruses between individuals of the chestnut blight fungus, Cryphonectria parasitica. Vegetative incompatibility inhibits horizontal virus transmission, but not completely. In an effort to assess the potential for the spread of hypoviruses in the Republic of Macedonia, we studied the transmission of Cryphonectria hypovirus 1 (CHV-1) among the five observed vegetative compatibility (vc) types of C. parasitica. One fungal isolate of each vc type was infected with CHV-1 and was paired in vitro with isolates of all other vc types for a total of 20 combinations of virus donors and recipients, and 250 replicate trials per combination. Virus transmission was scored after 7 days as successful if the recipient isolate took on an unpigmented culture phenotype typical of virus infection. Transmission occurred at high frequencies between some pairs of vc types, but in <1% of the trials for 10 of the 20 combinations of donors and recipients. Asymmetric transmission was observed between some vc types that had different alleles at vegetative incompatibility loci vic1 or vic7; i.e., transmission occurred at high frequencies in one direction, but very low frequencies between the same pair of isolates in the opposite direction. The expected virus transmission, calculated as the average transmission predicted for any two randomly chosen individuals from a population, was highly negatively correlated to vc type diversity. Results for isolates of C. parasitica from Macedonia were similar to those from Italy, but less transmission was generally observed. Differences in genetic background effects on transmission may vary among different populations even when isolates differ by the same vic alleles.
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- Horizontal transmission of hypoviruses between vegetative compatibility types of Cryphonectria parasitica in Macedonia
European Journal of Plant Pathology
Volume 120, Issue 1 , pp 35-42
- Cover Date
- Print ISSN
- Online ISSN
- Springer Netherlands
- Additional Links
- Biological control
- Chestnut blight fungus
- Vegetative incompatibility
- Virus transmission
- Industry Sectors
- Author Affiliations
- 1. Sumarski fakultet, Univerzitet “Sv. Kiril i Metodij”, 1000, Skopje, Republic of Macedonia
- 2. Istituto di Patologia Vegetale, Università degli Studi di Milano, 20133, Milan, Italy
- 3. Department of Plant Pathology, Cornell University, Ithaca, NY, 14853, USA