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Copper toxicity in a natural reference soil: ecotoxicological data for the derivation of preliminary soil screening values

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Abstract

The risk assessment of contaminated soils is conventionally done with the support of soil screening values (SSVs). Since SSVs are still unavailable for many European countries, including Portugal, standardized toxicity tests are urgently claimed for their derivation. Hence, this work aimed the generation of toxicity values for copper (Cu) in a natural reference soil (PTRS1) targeting different terrestrial species, endpoints and soil functions, as to derive a preliminary Cu SSV. For this, the Assessment Factor approach was applied, which allowed calculating predicted no effect concentrations (PNEC) for Cu that will be the basis for SSV proposal. In order to increase the reliability of the PNEC, and hence of the SSV, a lab/field factor was applied to correct the toxicity values used for PNEC determination. Cu affected urease, cellulase and nitrogen mineralization activities. The EC50 values calculated for the invertebrates reproduction were 130.9, 165.1 and 191.6 mg Cu Kg−1soildw for Eisenia andrei, Enchytraeus crypticus and Folsomia candida, respectively. Cu inhibited seed germination mainly for Lactuca sativa, whilst it was toxic for the growth of different plant species (EC50s between 89 and 290.5 mg Cu Kg−1soildw). Based on the outcomes gathered, we proposed SSVs for Cu ranging between 26.3 and 31.8 mg Kg−1 soildw, which is above the background values reported and below all the EC20s recorded for the species and endpoints herein analyzed. Overall, this work describes a procedure that could be easily followed by other European countries wishing to derive SSVs adjusted to their soils.

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Acknowledgments

Caetano A. L. was supported by a PhD Grant from FCT (Fundação para a Ciência e Tecnologia) (ref. SFRH/BD/48943/2008). Catarina R. Marques was supported by a Post-doctoral grant from FCT (ref. SFRH/BPD/47292/2008).

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Authors and Affiliations

  1. Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Ana Luísa Caetano, Catarina Ribeiro Marques & Fernando Gonçalves

  2. CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Ana Luísa Caetano, Catarina Ribeiro Marques & Fernando Gonçalves

  3. Department of Geosciences, University of Aveiro, GeoBioTec Research Center, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Eduardo Ferreira da Silva

  4. Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal

    Ruth Pereira

  5. CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, Rua dos Bragas, 289, 4050-123, Porto, Portugal

    Ruth Pereira

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Caetano, A.L., Marques, C.R., Gonçalves, F. et al. Copper toxicity in a natural reference soil: ecotoxicological data for the derivation of preliminary soil screening values. Ecotoxicology 25, 163–177 (2016). https://doi.org/10.1007/s10646-015-1577-7

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