Ecotoxicology

, Volume 20, Issue 4, pp 643–652

Abnormal development of motor neurons in perfluorooctane sulphonate exposed zebrafish embryos

  • Ling Zhang
  • Yuan-yuan Li
  • Tian Chen
  • Wei Xia
  • Yin Zhou
  • Yan-jian Wan
  • Zi-quan Lv
  • Geng-qi Li
  • Shun-qing Xu
Article

DOI: 10.1007/s10646-011-0604-6

Cite this article as:
Zhang, L., Li, Y., Chen, T. et al. Ecotoxicology (2011) 20: 643. doi:10.1007/s10646-011-0604-6

Abstract

Perfluorooctane sulfonate (PFOS) is an environmental organic pollutant, the potential neurotoxicity of which is causing great concern in fish. In the present study, we examined the effects of PFOS on motor neurons, and investigated the potential toxicological mechanisms oxidative stress in zebrafish embryos. Six-hour post-fertilization (hpf) zebrafish embryos were exposed to 1.0 mg/L PFOS, then we examined the expression of alpha-tubulin, proliferating cell nuclear antigen (PCNA), cyclin-dependent kinase 5 (CDK5), and peroxiredoxin 2 (PRX2) after PFOS exposure until 120 hpf. The results showed that PFOS increased alpha-tubulin in the coccygeal spinal cord (CSC) at 96 hpf, whereas decreased alpha-tubulin in the brain and spinal cord at 120 hpf. PCNA expression was highly increased in CSC and abdomen compared with control at 96 and 120 hpf after PFOS exposure. In addition, PFOS exposure caused CDK5 expression to be highly increased in brain region following by down-regulation of PRX2 expression at 96 hpf. These results indicated that, at least in part, the effect on motor neurons induced by PFOS was mediated by dynamically interfering with the expression of alpha-tubulin and PCNA. Furthermore, PFOS-induced toxicity was associated with oxidative stress by deregulating CDK5 and PRX2.

Keywords

Motor neuronsOxidative stressPFOSNeurodevelopmental toxicityZebrafish

Abbreviations

PFOS

Perfluorooctane sulfonate

PFCs

Perfluorooctanesulfonyl fluoride

PCNA

Proliferating cell nuclear antigen

CDK5

Cyclin-dependent kinase 5

PRX2

Peroxiredoxin 2

CSC

Coccygeal spinal cord

DMSO

Dimethyl sulfoxide

IHC

Immunohistochemistry

QPCR

Quantitative real-time PCR

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

LSD

Least significant difference

PE

Pericardium edema

CS

Curved spines

ED

Embryonic dissolution

BN

Brain necrosis

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ling Zhang
    • 1
    • 2
  • Yuan-yuan Li
    • 1
  • Tian Chen
    • 1
  • Wei Xia
    • 1
  • Yin Zhou
    • 1
    • 3
  • Yan-jian Wan
    • 1
  • Zi-quan Lv
    • 1
  • Geng-qi Li
    • 1
  • Shun-qing Xu
    • 1
  1. 1.Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Preventive Medicine, Medical College Wuhan University of Science and TechnologyWuhanChina
  3. 3.Microbiology & Biochemical Pharmacy Wuhan Polytechnic UniversityWuhanChina