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Genetic and morphologic diversity of Pseudophoxinus (Cyprinidae): implication for conservation in Anatolia

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Abstract

Pseudophoxinus a freshwater fish genus shows remarkable diversification with high number of endemic species in Anatolia. Most of the species of the genus are categorized as endangered for conservation in IUCN Red List due to water abstraction and habitat destruction. The aim of the study is to investigate genetic and morphological structure of Pseudophoxinus populations using allozyme, microsatellite markers and geometric morphometrics (eight landmarks). Sample collection was performed from eight localities for four different Pseudophoxinus species (P. Crassus, P. battalgili, P. egridiri, P. sp) in Anatolia in 2006. Genetic variation was assessed by using six microsatellite and ten allozyme loci. In total, 39 alleles for allozymes and 103 alleles for microsatellites were encountered in all populations. Mean observed heterozygosity was found in the range of 0.0143–0.0875 in allozymes and 0.146 to 0.463 in microsatellites. Significant morphological and genetic differentiation was measured among P. Crassus populations that may indicate to be considered different units in conservation action plans. There was no population differentiation detected by morphological and genetic measurements among P.battalgili populations. Bayesian individual assignment from Structure software revealed that P. battalgili populations are highly mixed in four sampling areas.

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Acknowledgments

This study was supported by The Scientific & Technological Research Council of Turkey (TÜBİTAK) and Middle East Technical University (METU). We are grateful to James Rohlf, Dean Adams, and Murat Aytekin for their advice and helpful comments about geometric morphometrics measurements, Fahrettin Küçük and Güler Ekmekçi for their help during field trip, Ergi Deniz Özsoy and Murat Yıldırım for their help in allozyme analyses. Special thanks to Donna Gordon for her revision.

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Authors and Affiliations

  1. Department of Biology, Abant İzzet Baysal University, 14280, Bolu, Turkey

    Telli Murat

  2. Department of Biology, Middle East Technical University, 06531, Ankara, Turkey

    Kence Aykut

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  1. Telli Murat
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  2. Kence Aykut
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Correspondence to Telli Murat.

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Equal contribution, Telli Murat and Kence Aykut

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Murat, T., Aykut, K. Genetic and morphologic diversity of Pseudophoxinus (Cyprinidae): implication for conservation in Anatolia. Environ Biol Fish 98, 571–583 (2015). https://doi.org/10.1007/s10641-014-0291-2

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  • DOI: https://doi.org/10.1007/s10641-014-0291-2

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