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P2Y1, P2Y2, and TRPV1 Receptors Are Increased in Diarrhea-Predominant Irritable Bowel Syndrome and P2Y2 Correlates with Abdominal Pain

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An Erratum to this article was published on 05 October 2016

Abstract

Background

Previous studies indicated that P2Y1 and P2Y2 receptors, which are widely distributed in the enteric nervous system, are related to pain, while TRPV1 may contribute to visceral pain and hypersensitivity states in irritable bowel syndrome (IBS). Other studies showed that ATP activates the capsaicin-sensitive TRPV1 channel via P2Y receptors.

Aim

To detect the expression of P2Y1, P2Y2, and TRPV1 receptors in diarrhea-predominant IBS (IBS-D) patients and analyze any correlations with abdominal pain and to investigate interactions between P2Y receptors and the TRPV1 receptor in IBS-D patients.

Methods

Rectosigmoid biopsies were collected from patients with IBS-D (n = 36) and healthy controls (n = 15). Abdominal pain was scored using a 10-cm visual analogue scale. Expression levels of P2Y1, P2Y2, and TRPV1 receptors in rectosigmoid biopsies were determined by real-time PCR and double-labeling immunofluorescence with specific antibodies.

Results

Both mRNA and protein expression levels of P2Y1, P2Y2, and TRPV1 receptors were increased in IBS-D compared with controls. Of these receptors, P2Y2 expression correlated with the maximum pain scores (p = 0.02, r = 0.63, Spearman correlation) in IBS-D patients. However, no relationships were detected between P2Y receptors and the TRPV1 receptor.

Conclusion

In the present study, we identified an increased expression of P2Y1 and P2Y2 receptors in the rectosigmoid mucosa of IBS-D patients, and P2Y2 correlated with abdominal pain. Furthermore, we identified an increase in TRPV1 expression; however, there were no correlations found between P2Y receptors and the TRPV1 receptor.

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Acknowledgments

This study is supported by a grant from MIMS (Shanghai) Ltd. of China (Grant Number: IDF-2013-07). We gratefully thank the staffs of the Department of Gastroenterology, The Second Affiliated Hospital of Xi’an Jiaotong University. The staffs of the Central Laboratory of the Second Affiliated Hospital of Xi’an Jiaotong University are thanked for their technical assistance.

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Authors and Affiliations

  1. Department of Gastroenterology, The Second Affiliated Hospital of Xi’an Jiaotong University, No. 157, Xi Wu Road, Xi’an, 710004, Shanxi, China

    Yumei Luo, Cheng Feng, Jing Wu, Dong Liu, Fei Dai & Jun Zhang

  2. Department of Pathology, The Second Affiliated Hospital of Xi’an Jiaotong University, No. 157, Xi Wu Road, Xi’an, 710004, Shanxi, China

    Jie Wu

  3. Department of Neurosurgery, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277, Yanta West Road, Xi’an, 710061, Shanxi, China

    Yongxing Wu

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  1. Yumei Luo
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Correspondence to Jun Zhang.

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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s10620-016-4320-1.

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Luo, Y., Feng, C., Wu, J. et al. P2Y1, P2Y2, and TRPV1 Receptors Are Increased in Diarrhea-Predominant Irritable Bowel Syndrome and P2Y2 Correlates with Abdominal Pain. Dig Dis Sci 61, 2878–2886 (2016). https://doi.org/10.1007/s10620-016-4211-5

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