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High Fat Diet Differentially Regulates the Expression of Olfactory Receptors in the Duodenum of Obesity-Prone and Obesity-Resistant Rats

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Abstract

Background

The gastrointestinal tract is important in the regulation of food intake, nutrient sensing and nutrient absorption. Obesity-prone Osborne-Mendel (OM) rats are less sensitive to the satiating effects of a duodenal infusion of fatty acids than obesity-resistant S5B/Pl (S5B) rats, suggesting that the gastrointestinal tract differentially senses the presence of fat in these two strains. A microarray analysis was conducted to identify genes that were differentially expressed in the duodenal enterocytes of OM and S5B rats.

Aims

The present experiment evaluated the expression of olfactory receptors in the duodenal enterocytes of OM and S5B rats. It was hypothesized that olfactory receptors present in the duodenum of OM and S5B rats would be differentially regulated by the intake of a high fat diet.

Methods

The mRNA levels of four olfactory receptors (Olr1744, Olr50, Olr124, Olr1507) were assessed from the duodenal enterocytes of OM and S5B rats consuming a high fat diet for 14 days.

Results

The duodenal mRNA levels of Olr1744, Olr124 and Olr1507 were significantly elevated in OM rats fed the high fat diet, but not S5B rats. No differences in the expression of Olr50 receptor mRNA were detected.

Conclusions

These data suggest that several olfactory receptors present in the duodenum are selectively regulated by high fat diet intake in obesity-prone OM rats. Therefore, these receptors may play a role in the sensing and regulation of dietary fat, and may be important for the individual susceptibility to obesity in these two strains.

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Acknowledgments

This research was supported by NIDDK 32089 to G. A. Bray. The authors would like to thank Eli Bench, Christine Blackmon, Katherine Pyburn and Raphael Malbrue for their assistance on this project. This work was supported in part by NINDS49177 to G.M. Holmes, P20-RR021945 from the National Center for Research Resources and NIH NORC (CNRU) Center Grant 1P30 DK072476.

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Authors and Affiliations

  1. Joint Diabetes, Endocrinology and Metabolism Program, Louisiana State University System, Louisiana State University Health Science Center-New Orleans (LSUHSC-NO), New Orleans, LA, 70112, USA

    Stefany D. Primeaux

  2. Pennington Biomedical Research Center, Baton Rouge, LA, 70808, USA

    H. Douglas Braymer & George A. Bray

  3. Internal Medicine-Endocrinology, Diabetes and Metabolism, Louisiana State University System, Louisiana State University Health Science Center-New Orleans (LSUHSC-NO), 1542 Tulane Ave, Box T4 M-2, New Orleans, LA, 70112, USA

    Stefany D. Primeaux

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  1. Stefany D. Primeaux
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  2. H. Douglas Braymer
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  3. George A. Bray
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Correspondence to Stefany D. Primeaux.

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Primeaux, S.D., Braymer, H.D. & Bray, G.A. High Fat Diet Differentially Regulates the Expression of Olfactory Receptors in the Duodenum of Obesity-Prone and Obesity-Resistant Rats. Dig Dis Sci 58, 72–76 (2013). https://doi.org/10.1007/s10620-012-2421-z

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  • DOI: https://doi.org/10.1007/s10620-012-2421-z

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