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Protective Effects of Melatonin and Octreotide Against Radiation-Induced Intestinal Injury

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Abstract

Purpose

To compare the protective effects of the potent antioxidants, melatonin and octreotide, against radiation-induced intestinal injury.

Methods

A total of 42 male 3-month-old Swiss albino mice (40 ± 10 g) were matched according to body weight and randomly assigned to one of six groups: control; radiation treatment (RT) only; melatonin only (15 mg/kg, i.p.); melatonin + RT; octreotide only (50 μg/kg i.p.); and octreotide + RT. Intestinal damage was induced by exposure to a single whole-body radiation dose of 8 Gy. All mice tolerated the experimental interventions, and no deaths were observed.

Results

Irradiation induced architectural disorganization, including inflammatory mononuclear cell infiltration, villitis, and desquamation with eosinophilic necrosis, and diminished mucosal thickness, crypt height, and villous height. In the melatonin + RT and octreotide + RT groups, the villous pattern was well preserved; desquamation at villous tips and edema was prominent, but necrosis was absent. The radiation-induced decrease in mucosal thickness was significantly reduced by pretreatment with melatonin (p < 0.001) or octreotide (p = 0.01), although the protective effect was significantly greater for melatonin (p = 0.04). Pretreatment with melatonin also preserved villous height (p = 0.009) and crypt height (p = 0.03); although a similar trend was observed for pre-irradiation octreotide, the differences were not significant.

Conclusions

Melatonin and octreotide potently protected against radiation-induced intestinal injury in mice, but melatonin was significantly more effective in preserving the histological structure of the intestines, a finding that warrants confirmation in clinical studies.

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Acknowledgments

The authors wish to thank Assoc. Prof. Dr. S. Remzi Erdem, MD, PhD, Sebnem Kosdak, MD and Hande Ozge Altunkaynak MSc, from the Department of Pharmacology, Baskent University Faculty of Medicine, for their constructive criticism on the experimental design of the study and valuable help in preparing the drug solutions. This study is accepted as poster presentation at ECCO 15/ESMO 34 - Berlin, September 20–24, 2009.

Conflict of interest

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Authors and Affiliations

  1. Department of Radiation Oncology, Adana Research and Treatment Centre, Baskent University Medical Faculty, 01120, Adana, Turkey

    Cem Onal, Erkan Topkan, Melek Yavuz & Aydin Yavuz

  2. Department of Pathology, Baskent University Medical Faculty, Ankara, Turkey

    Fazilet Kayaselcuk

  3. Experimental Animals Breeding and Research Center, Baskent University Medical Faculty, Baglica, Ankara, Turkey

    Didem Bacanli

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  1. Cem Onal
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Correspondence to Cem Onal.

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Onal, C., Kayaselcuk, F., Topkan, E. et al. Protective Effects of Melatonin and Octreotide Against Radiation-Induced Intestinal Injury. Dig Dis Sci 56, 359–367 (2011). https://doi.org/10.1007/s10620-010-1322-2

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  • DOI: https://doi.org/10.1007/s10620-010-1322-2

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