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Association of MicroRNA-196a-2 Gene Polymorphism with Gastric Cancer Risk in a Chinese Population

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Abstract

Background

It has been proposed that single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) could affect the expression of the miRNA and contribute to the susceptibility of human tumors. However, the role of genetic variant (T/C) in miR-196a-2 in gastric cancer susceptibility is still unknown.

Objectives

To evaluate the association between genetic polymorphism of miR-196a-2 (rs11614913) and risk of gastric cancer, a hospital-based case–control study was conducted in a Chinese population.

Methods

The miR-196a-2 polymorphism was determined using the method of polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) in 213 gastric cancer patients and 213 age- and sex-matched controls.

Results

In the present study, we found that a significantly increased risk of gastric cancer in subjects with the variant homozygote CC of miR-196a-2 compared with wild-type homozygote TT and heterozygote CT carriers (adjusted odds ratio (OR) = 1.57, 95% confidence interval (CI) = 1.03–2.39, P = 0.038). Stratified analyses indicated that the variant homozygote CC genotype had a strong association with lymph node metastasis of gastric cancer (adjusted OR = 2.25, 95% CI = 1.21–4.18, P = 0.011).

Conclusions

These findings suggest that the genetic variant within miR-196a-2 could play an important role in the development and progression of gastric cancer. We expect the findings may be helpful to better understand the mechanism of gastric carcinogenesis.

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Abbreviations

CI:

Confidence interval

OR:

Odds ratio

PCR:

Polymerase chain reaction

miRNAs:

microRNAs

SNP:

Single nucleotide polymorphism

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Acknowledgments

This work was supported by grants from the Science Foundation of the Health Bureau, Wuxi, PR China (No. XM0805); Special Foundation for High Degree Talents of Shihezi University, China (No. RCZX200686).

Conflict of Interest Statement

None declared.

Author information

Authors and Affiliations

  1. Department of Anesthesiology, The Fourth Affiliated Hospital of Soochow University, 214062, Wuxi, People’s Republic of China

    Sheng Peng & Yan Zhang

  2. Intensive Care Unit of the First Affiliated Hospital of Medical College, Shihezi University, 832008, Shihezi, People’s Republic of China

    Zhongshen Kuang, Hang Xu & Qinghong Cheng

  3. Department of General Surgery, Affiliated Hospital of Nantong University, 226001, Nantong, People’s Republic of China

    Chenyi Sheng

Authors
  1. Sheng Peng
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  2. Zhongshen Kuang
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  3. Chenyi Sheng
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  4. Yan Zhang
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  5. Hang Xu
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  6. Qinghong Cheng
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Corresponding author

Correspondence to Sheng Peng.

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Peng, S., Kuang, Z., Sheng, C. et al. Association of MicroRNA-196a-2 Gene Polymorphism with Gastric Cancer Risk in a Chinese Population. Dig Dis Sci 55, 2288–2293 (2010). https://doi.org/10.1007/s10620-009-1007-x

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  • DOI: https://doi.org/10.1007/s10620-009-1007-x

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