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Probiotics and Blueberry Attenuate the Severity of Dextran Sulfate Sodium (DSS)-Induced Colitis

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Abstract

We studied the anti-inflammatory properties of probiotic strains and blueberry in a colitis model. The disease activity index (DAI) was significantly lower on days 9 and 10 in all groups compared to the colitis control. Myeloperoxidase (MPO) and bacterial translocation to the liver and to the mesenteric lymph nodes (MLN) decreased significantly in all groups compared to colitis control. Cecal Enterobacteriaceae count decreased significantly in blueberry with and without probiotics compared to the other groups. Lactobacillus plantarum reisolated from the cecal content in the presence of blueberry, contrary to Lactobacillus fermentum. Colonic MDA decreased significantly in all groups, except the L. fermentum group, compared to the colitis control. The cecal concentration of acetic, propionic, and butyricbutyric acid was significantly higher in the L. plantarum group, while the L. fermentum group yielded the highest concentration of lactic acid compared with all other groups. Lactobacillus plantarum DSM 15313, Lactobacillus fermentum 35D, and blueberry alone and in combination improve the DAI, reduce bacterial translocation, and reduce inflammation.

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Acknowledgments

This work was carried out with financial support from VINNOVA, Swedish Governmental Agency for Innovation Systems. It is also supported by grant no. K2000-72X-11616-04C from the Swedish Medical Research Council, Dir. A. Påhlsson’s Foundation, Ruth and Richard Julin’s Foundation, Malmö University Hospital, Lundgren’s Foundation, Gunnar Nilsson’s Foundation, and Apotekaren Hedberg’s Fond.

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Authors and Affiliations

  1. Food Hygiene, Department of Food Technology, Engineering and Nutrition, Lund University, Malmo, Sweden

    Nadia Osman, Siv Ahrné & Göran Molin

  2. Department of Surgery, University Hospital Malmö, Lund University, 205 02, Malmo, Sweden

    Diya Adawi & Bengt Jeppsson

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  1. Nadia Osman
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  2. Diya Adawi
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  5. Göran Molin
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Correspondence to Diya Adawi.

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Osman, N., Adawi, D., Ahrné, S. et al. Probiotics and Blueberry Attenuate the Severity of Dextran Sulfate Sodium (DSS)-Induced Colitis. Dig Dis Sci 53, 2464–2473 (2008). https://doi.org/10.1007/s10620-007-0174-x

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