Original Article

Digestive Diseases and Sciences

, Volume 52, Issue 10, pp 2725-2731

Demonstration of Low-Regulatory CD25High+CD4+ and High-Pro-inflammatory CD28CD4+ T-Cell Subsets in Patients with Ulcerative Colitis: Modified by Selective Granulocyte and Monocyte Adsorption Apheresis

  • Yoko YokoyamaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Ken FukunagaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine Email author 
  • , Yoshihiro FukudaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Katsuyuki TozawaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Koji KamikozuruAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Kunio OhnishiAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Takeshi KusakaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Tadashi KosakaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
  • , Nobuyuki HidaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
    • , Yoshio OhdaAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
    • , Hiroto MiwaAffiliated withDivision of Upper Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine
    • , Takayuki MatsumotoAffiliated withDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

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Abstract

Low-CD25High+CD4+, a subset of regulatory CD25+CD4+ T cells and high-inflammatory CD28CD4+ T cells can exacerbate ulcerative colitis (UC). This study sought to investigate the frequency of CD25High+CD4+ and CD28CD4+ T cells in patients with UC and the changes in these cells during Adacolumn granulocyte and monocyte adsorption apheresis (GMA). Subjects were 12 patients with active UC, 11 with quiescent UC, and 14 healthy volunteers (HVs). The mean clinical activity index was 15.7 ± 2.2 in active UC and 4.5 ± 1.1 in quiescent UC. Peripheral blood samples were stained with CD4, CD25, and CD28 antibodies for flow cytometry. Patients with active UC received GMA and blood samples were examined before and after the first GMA session. Patients with active UC (P < 0.04) or quiescent UC (P < 0.02) had a higher percentage of CD28D4+T cells compared with HVs, while the percentage of CD28+CD4+ T cells was lower in both UC groups compared with HVs (P = 0.03 and P < 0.02). Patients with active UC had a lower percentage of CD25High+CD4+T cells compared with quiescent UC patients (P < 0.001). A significant increase in CD25High+CD4+ T cells was associated with GMA (P < 0.03). Low CD25High+CD4+ and high CD28CD4+ are prominent features in UC. The increase in CD25High+CD4+ T cells induced by GMA should contribute to improved immune function. Additional studies are warranted, since a low frequency of CD25High+CD4+ and a high frequency of CD28CD4+ expressing T cells might be a predictor of clinical response to GMA.

Keywords

Ulcerative colitis Regulatory T cells CD25High+CD4+ CD28+CD4+ CD28CD4 Granulocyte and monocyte apheresis