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Regenerative effect of hOPG gene-modified autologous PDLs in combination with cell transplantation on periodontal defection in beagle dogs

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Abstract

This study evaluated the ability of human osteoprotegerin gene-modified autologous periodontal ligament cells (PDLCs) in combination with cell transplantation to promote periodontal regeneration in beagle dogs. Adenovirus Ad5-hOPG-EGFP-transfected PDLCs and BME-10X collagen membranes were fabricated and used for periodontal repair. Buccal periodontal defects (mesiodistal width × depth: 5 × 5 mm) were created on the second, third, and fourth mandibular premolars in six normal beagle dogs, and the defects were histologically and histomorphometrically assessed for periodontal regeneration in the following four groups: (1) hOPG-PDLCs + BME-10X, (2) mock-PDLCs + BME-10X, (3) PDLCs + BME-10X, and (4) BME-10X. The radiographic and histological results suggested that hOPG-PDLCs significantly promoted periodontal defect repair. This study demonstrates the potential of hOPG-modified PDLCs for periodontal tissue regeneration.

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Acknowledgments

This study was supported by the International Cooperation Research and Develop Project of Nanjing Health Bureau (No. 201303051), “Six Talent Peaks” of High Level Talent Selection and Training Project of Jiangsu Province (No. 2013-SWYY-006), Research Fund for the Doctoral Program of Higher Education of China (No. 20093518110002) and Key Project of Science and Technology Bureau of Jiangsu Province (No. BL2013002).

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Authors and Affiliations

  1. Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China

    Su Jiang, Bin Chen & Fuhua Yan

  2. School of Stomatology Fujian Medical University, Fuzhou, 350002, Fujian, China

    Kunqi Tang

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  1. Su Jiang
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  2. Kunqi Tang
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  3. Bin Chen
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  4. Fuhua Yan
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Correspondence to Fuhua Yan.

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Jiang, S., Tang, K., Chen, B. et al. Regenerative effect of hOPG gene-modified autologous PDLs in combination with cell transplantation on periodontal defection in beagle dogs. Cytotechnology 68, 2613–2623 (2016). https://doi.org/10.1007/s10616-016-9985-y

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  • DOI: https://doi.org/10.1007/s10616-016-9985-y

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