Abstract
IL-37 has been described as a natural inhibitor of immune responses. Monoclonal antibody (mAb) against human IL-37b with high affinity and specificity can serve as a molecular probe to detect IL-37 and study IL-37 functions, mechanisms and related signal pathways in inflammatory diseases. However, there are very few such mAbs against human IL-37 commercially available so far. In the current study, monoclonal antibodies against human IL-37b were developed by fusing splenocytes from immunized mouse with SP2/0 myeloma cells and polyethylene glycol. Then the antibodies were screened with prokaryotic expressed human IL-37b protein and eukaryotic expressed human IL-37b protein subsequently. Western blot and flow cytometry analysis revealed that selected mAb clons were able to recognize human IL-37 with high specificity. And more importantly, the IL-37b mAbs were fluorescently labeled and can be directly used in flow cytometry and immunohistochemistry. In conclusion, the current study developed new mAbs against human IL-37b, which are applicable in flow cytometry and immunohistochemistry.
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Acknowledgements
This work was supported in part by grants from the National Natural Science Foundation of China (81273237 and 81570009), Natural Science Foundation of Guangdong Province (2015A030313513), Social science and Technology Project of Dongguan city (201550715000314), all to JFX.
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Yu-Chi Gao, Yan Jia, De-Qian Xiao have contributed equally to this work.
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Gao, YC., Jia, Y., Xiao, DQ. et al. Development and characterization of monoclonal antibody against human IL-37b. Cytotechnology 69, 217–227 (2017). https://doi.org/10.1007/s10616-016-0052-5
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DOI: https://doi.org/10.1007/s10616-016-0052-5