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Oncogenic Ras influences the expression of multiple lncRNAs

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Abstract

Recent ultrahigh-density tiling array and large-scale transcriptome analysis have revealed that large numbers of long non-coding RNAs (lncRNAs) are transcribed in mammals. Several lncRNAs have been implicated in transcriptional regulation, organization of nuclear structure, and post-transcriptional processing. However, the regulation of expression of lncRNAs is less well understood. Here, we show that the exogenous and endogenous expression of an oncogenic form of small GTPase Ras (called oncogenic Ras) decrease the expression of lncRNA ANRIL (antisense non-coding RNA in the INK4 locus), which is involved in the regulation of cellular senescence. We also show that forced expression of oncogenic Ras increases the expression of lncRNA PANDA (p21 associated ncRNA DNA damage activated), which is involved in the regulation of apoptosis. Microarray analysis demonstrated that expression of multiple lncRNAs fluctuated by forced expression of oncogenic Ras. These findings indicate that oncogenic Ras regulates the expression of a large number of lncRNAs including functional lncRNAs, such as ANRIL and PANDA.

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Acknowledgments

We greatly appreciate Dr. T Kitamura (The University of Tokyo, Tokyo, Japan) and Dr. CJ Der (University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) for providing the plasmids used in this study. This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to YK, KK, HN, and MK) and Takeda Science Foundation (to YK).

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Authors and Affiliations

  1. Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka, 820-8555, Japan

    Yojiro Kotake & Madoka Naemura

  2. Department of Molecular Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan

    Kyoko Kitagawa, Hiroyuki Niida & Masatoshi Kitagawa

  3. Department of Cell Biology, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan

    Toshiyuki Tsunoda & Senji Shirasawa

Authors
  1. Yojiro Kotake
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  2. Madoka Naemura
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  3. Kyoko Kitagawa
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  4. Hiroyuki Niida
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  5. Toshiyuki Tsunoda
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  7. Masatoshi Kitagawa
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Correspondence to Yojiro Kotake.

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Kotake, Y., Naemura, M., Kitagawa, K. et al. Oncogenic Ras influences the expression of multiple lncRNAs. Cytotechnology 68, 1591–1596 (2016). https://doi.org/10.1007/s10616-014-9834-9

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  • DOI: https://doi.org/10.1007/s10616-014-9834-9

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