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Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells

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Abstract

Deleterious invasiveness of glioma cells into the normal brain tissue is endorsed by its inherent ability to regulate the receptor-mediated adhesive properties, extracellular matrix degradation and remodeling and elevated secretory ability of metalloproteinase (MMPs) such as MMP-2. By doing so, it will create an intercellular space for the invasion of glioma cells. Here, we reported that combination of gene therapy Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, with lithium chloride (LiCl), clinically used as mood stabilizer, could inhibit the migration and invasion of C6 glioma cells. The results showed that concomitant administration of LiCl and pEGFP-N1-BmK CT on glioma cells would hamper pro-MMP2 secretion and in the meantime, inhibited its proliferation in a synergistic manner. These results try to extrapolate the potential interplay between the combined treatment of LiCl and BmK CT with signaling pathways β-catenin, MMP, GSK-3 in C6 glioma cells. This strategy can stand for a novel approach designated for the development of a new method for glioma therapy.

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Acknowledgments

This project was supported by grants from ‘National Natural Science Foundation of China (Nos. 31272100 and 31372199)’, ‘Natural Science Foundation of Shanxi Province (No. 2014011038-1)’, ‘National High Technology Research and Development Program of China (863 Program, No. 2012AA020809)’, and ‘the Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi’.

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Yuejun Fu, Yanmei Jiao & Aihua Liang

  2. University of Miami Miller School of Medicine, Miami, FL, 33136, USA

    Shuhua Zheng

  3. Department of Neurology, Shanxi Provincial People’s Hospital, Taiyuan, 030012, People’s Republic of China

    Fengyun Hu

Authors
  1. Yuejun Fu
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  2. Yanmei Jiao
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  3. Shuhua Zheng
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  4. Aihua Liang
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  5. Fengyun Hu
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Correspondence to Yuejun Fu or Fengyun Hu.

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Fu, Y., Jiao, Y., Zheng, S. et al. Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells. Cytotechnology 68, 197–202 (2016). https://doi.org/10.1007/s10616-014-9768-2

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  • DOI: https://doi.org/10.1007/s10616-014-9768-2

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