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Adaptation and cultivation of permanent fish cell line CCO in serum-free medium and influence of protein hydrolysates on growth performance

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Abstract

In this work we describe the adaptation of channel catfish ovary (CCO) cell line to commercially available Ultra Culture serum-free medium by gradual reduction of serum concentration from 10 to 0 %. With this approach we obtained CCO cells fully adapted to serum-free conditions in 32 days. Growth, nutritional and morphological characteristics of these cells remained unchanged when compared to the control group kept in the presence of serum. Additionally, three commercially available protein hydrolysates were tested for the effects on growth performance of the newly serum-free adapted CCO cells. Supplementation with wheat gluten hydrolysate resulted in growth similar to serum free medium solely, while yeast and soy hydrolysates showed inhibitory effects on the cell growth. Taken together, the successful adaptation of CCO cells to serum-free conditions indicates their potential to be used in cytotoxicity assays when serum omission is demanded or for developing serum free bioprocesses using CCO cells. However, a more extended study on nutrient supplementation is still required to further boost the cell growth in a serum free culture.

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Acknowledgments

Support from the Ministry of Science, Education and Sports, Republic of Croatia (Grant No. 058-0582184-2414) is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Cell Culture Technology and Biotransformation, Faculty of Food Technology and Biotechnology, University of Zagreb, 6 Pierotti St., 10000, Zagreb, Croatia

    Kristina Radošević, Bogdanka Dukić, Martina Andlar, Igor Slivac & Višnja Gaurina Srček

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  1. Kristina Radošević
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  2. Bogdanka Dukić
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  3. Martina Andlar
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  4. Igor Slivac
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  5. Višnja Gaurina Srček
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Correspondence to Višnja Gaurina Srček.

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Radošević, K., Dukić, B., Andlar, M. et al. Adaptation and cultivation of permanent fish cell line CCO in serum-free medium and influence of protein hydrolysates on growth performance. Cytotechnology 68, 115–121 (2016). https://doi.org/10.1007/s10616-014-9760-x

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