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Genotoxicity of indium tin oxide by Allium and Comet tests

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Abstract

Genotoxic effects of indium tin oxide (ITO) were investigated on root cells of Allium cepa by employing both Allium and Comet assays. A. cepa roots were treated with the aqueous dispersions of ITO at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of ITO significantly increased mitotic index, and total chromosomal aberrations by the Allium test. While chromosome laggards, stickiness, disturbed anaphase–telophase and anaphase bridges were observed in anaphase–telophase cells, c-metaphase and binuclear cells were observed in other cells. A significant increase in DNA damage was also observed at all concentrations of ITO by the Comet assay. These results indicate that ITO exhibits genotoxic activity in A. cepa root meristematic cells.

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Authors and Affiliations

  1. Biology Department, Faculty of Science and Literatures, Afyon Kocatepe University, 03200, Afyon, Turkey

    İbrahim Hakkı Ciğerci & Emre Özgül

  2. Molecular Biology and Genetics Department, Faculty of Arts and Sciences, Uşak University, 1 Eylül Campus, 64300, Uşak, Turkey

    Recep Liman

  3. Molecular Biology and Genetics Department, Faculty of Engineering and Natural Sciences, Üsküdar University, Altunizade, 34662, Istanbul, Turkey

    Muhsin Konuk

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  1. İbrahim Hakkı Ciğerci
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  2. Recep Liman
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  3. Emre Özgül
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  4. Muhsin Konuk
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Correspondence to Recep Liman.

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Ciğerci, İ.H., Liman, R., Özgül, E. et al. Genotoxicity of indium tin oxide by Allium and Comet tests. Cytotechnology 67, 157–163 (2015). https://doi.org/10.1007/s10616-013-9673-0

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